不同针刺刺激量对寒凝证类痛经模型大鼠扭体反应及子宫内前列腺素物质含量的影响  

Effect of Different Acupuncture Stimulus on Writhing Response and Prostaglandin Content of Dysmenorrhea Rats with Cold Syndrome

作　　者：齐丹丹[1] 张玲[1] 申松希[1] 朱世鹏[1] 沈小雨[1] 许咏思[1] 杨佳敏[1] 罗丽 任晓暄[1,2] 郭孟玮[1,2] 赵雅芳[1,2] 李晓泓[1,3] 嵇波[1,2,3] 张露芬[1,2] 朱江[1,3] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]国家中医药管理局针灸生物学三级实验室,北京100029 [3]国家中医药管理局针灸特色疗法评价重点研究室,北京100029

出　　处：《中医药信息》2015年第4期48-51,共4页Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(NO.2012CB518506;2006CB504503);国家重点基础研究发展计划(973计划)(NO.2005CB523308);国家自然科学基金面上项目(NO.30973793);教育部博士点基金项目(NO.20090013110005)

摘　　要：目的:观察不同针刺刺激量干预三阴交穴后对模型大鼠扭体反应及子宫组织内前列腺素含量的影响,探讨不同针刺"得气"状态下部分镇痛机制。方法:按随机数字法,将36只动情间期SD雌鼠分为盐水对照组、寒凝证类痛经模型组(模型组)、刺激量A组(期望"得气"组)与刺激量B组(期望"不得气"组),每组9只。盐水组每日给予生理盐水,其余均采用全身冷冻加雌二醇注射法制备模型,造模成功后刺激量A组采用粗针深刺行手法诱导动物"得气"状态;刺激量B组采用细针浅刺不行手法避免"得气",观察不同针刺刺激量干预下大鼠每分钟扭体反应,并于留针后20min取大鼠子宫组织采用酶联免疫吸附法检测PGF2α、PGE2含量的变化。结果:1)每分钟扭体反应评分:与盐水组比较,模型组扭体评分显著增高(P<0.01);与模型组比较,期望"得气"组扭体评分显著降低(P<0.01),与期望"得气"组比较,期望"不得气"组每分钟扭体评分显著增高(P<0.01)。2)PGF2α、PGE2含量:与盐水组比较,各组PGF2α含量显著增多,期望"不得气"组PGE2含量显著增多(P<0.01,P<0.05),模型组与期望"得气"组PGF2α/PGE2比值显著增多。与模型组和期望"得气"组比较,期望"不得气"组PGE2含量增高。结论:针刺三阴交穴可缓解大鼠扭体反应,期望"得气"组疗效显著,期望"不得气"组疗效不及期望"得气"组,相应分子响应机制认为,期望"不得气"组缓解痛经的基础机制可能是通过调节子宫组织内前列腺素E2水平及使PGF2α/PGE2趋于平衡,进而缓解子宫平滑肌痉挛而达到镇痛效果。Objective：To investigate the writhing response of each minute and the synthesis of prostanoids（ PGs） of uterine on experimental cold syndrome of primary dysmenorrheal model rats by using different acupuncture methods at SP6.Methods：36 female SD rats were randomized into 4 groups,including saline control group,cold stagnation of primary dysmenorrheal model control group（ model control group） ,A stimuli group（ Deqi group） and B stimuli group （no Deqi group）,with 9 rats in each group.Except the saline control group,in the rest groups rats were treated with the whole body freezing method combined with estradiol benzoate injection mold, and in the saline control group rats were injected 0.9%Nacl.Then the acupuncture groups acupunctured the corresponding point for 20min after modeling,A stimuli group needled bilateral SP6 acupoint with the rats lying face up,in order to induce needing sensation（ Deqi）;B stimuli group needled the bilateral SP6 acupoint with the rats lying face up,puncturing vertically with one 0.18mm ×13mm stainless,sterilized and disposable to a depth of 1～2mm,maintaining the needle in place for 20 minutes.Observe the writhing response and the PGE2 and PGF2αin uterine of 9 rats in each group by ELISA.Results：Compared with the model control group and B stimuli group,the writhing scores in each minute were decreased significantly in a stimuli group （ P〈0 .01 ） . Conclusion：Manual acupuncture at SP6 can relieve the pain of dysmenorrhea and A stimuli group has a better effect than other groups.

关 键 词：痛经 针刺刺激量 得气 扭体评分 PGF2Α PGE2 

分 类 号：R245[医药卫生—针灸推拿学]