干扰高迁移率族蛋白A2的表达对NB4细胞裸鼠种植瘤生长的影响  

Effect of HMGA2 knockdown on cell growth in NB4 cell xenograft tumor

作　　者：魏小平[1] 谭丽[1] 潘庆珍 林伟尚 

机构地区：[1]广州医科大学附属第一医院,510120 [2]广州荔湾区妇幼保健院,510375 [3]广州市番禺钟村医院,511495

出　　处：《实用医学杂志》2015年第15期2437-2439,共3页The Journal of Practical Medicine

基　　金：广东省科技厅项目(编号:2013B22000102);广东省医学科研基金项目(编号:A2014292)

摘　　要：目的：建立裸鼠体内NB4／ShHMGA2细胞种植瘤模型。探索稳定敲低高迁移率族蛋白A2（HMGA2）的表达对白血病细胞生物学形状的影响。方法：每组5只4。5周龄的BALB／c裸鼠．经4Gy60C0照射后腋窝皮下分别接种8×10^6个NB4／ShHMGA2、NB4／ShControl细胞（对照组），观察成瘤性。3周后处死裸鼠。免疫组化法检测NB4细胞种植瘤组织切片中Ki．67蛋白的表达水平。结果：NB4／shHMGA2及对照细胞在裸鼠腋下均成瘤，NB4／ShHMGA2细胞肿瘤生长速度明显慢于对照组，NB4／ShHMGA2细胞与对照组瘤体体积分别为（1484．25±156．342）和（3228．674±285．64）mm^3，差异有统计学意义（P〈0．05）：NB4／ShHMGA2细胞与对照组瘤块重量分别为（650．46±85．12）和（2135．33±198．05）mg，差异有统计学意义（P〈0．05）。免疫组化检测显示NB4／ShHMGA2细胞组种植瘤组织切片Ki-67蛋白表达水平低于对照组。结论：敲低HMGA2的表达在体内可抑制白血病细胞的增殖。Objective To establish a nude mice model for NB4/ShHMGA2 xenograft and explore the effect of HMGA2 knockdown on hematological malignancies. Methods NB4/ShHMGA2 or NB4/ShControl cell lines were established by transfecting the recombinant Lentivirus-HMGA2shRNA and the vacant Lentivirus-NC-marked into NB4 cells. The knockdown of HMGA2 was identified by RT-PCR and Western blot. Ten male BALB/c nude mice aged 4 ~ 5 weeks were equally divided into two groups. The mice irradiated by 4 Gy 60 Co were subcutaneously injected with 8 × 10^6 NB4/ShHMGA2 or NB4/ShControl cells into one side of axilla. The volumes of xenograft tumor were evaluated using the equation volume （mm^3） = （L × W2）/2. The xenograft tumor section was detected by IHC with Ki-67 antibody. Results NB4 cell xenograft tumors developed in all mice of both the two groups. The NB4/ShHMGA2 cells in the nude mice grew at a lower rate than those in the controls. There were statistically significant differences in the volume and weight of xenograft tumor between the two groups [ （ 1 484.25 ±156.342）mm^3 vs （3 228.674 ± 285.64）mm^3, P 〈 0.05] and [（2 135.33 ± 198.05） mg vs （650.46 ±85.12）mg, P 〈 0.05]. The Ki-67 protein level in NB4/ShHMGA2 cells xenografts was lower than that in the controls. Conclusion The knockdown of HMGA2 could inhibit proliferation of NB4 cells in NB4 cells xenograft tumor.

关 键 词：HMGA2 NB4细胞:细胞增殖 

分 类 号：R73-36[医药卫生—肿瘤]