ELISA法检测血清中CD44v5含量用于食管鳞癌筛检的初步研究  

Detection the CD44v5 in serum for screening ESCC through ELISA

作　　者：王兴名 王洪江[1] 张园 王勇涛[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胸外科,乌鲁木齐市830011 [2]新疆肿瘤防治研究所,乌鲁木齐市830011

出　　处：《实用医学杂志》2015年第14期2248-2252,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81160271)

摘　　要：目的:检测食管鳞癌患者CD44v5表达情况并分析其与临床病理特征之间的关系,探讨血清中CD44v5含量检测用于食管鳞癌筛检的应用价值。方法:采用RT-PCR技术和Western-Blot法分别检测CD44v5基因在食管鳞癌组织及癌旁组织中的表达,并采用酶联免疫吸附法(ELISA法)测定该蛋白在100例食管鳞癌患者及60例健康体检者血清中的含量。结果:(1)CD44v5在食管鳞癌组织中的相对表达量高于癌旁食管组织,且CD44v5表达在不同TNM分期、癌组织浸润深度、有无淋巴结转移及分化程度中存在差异(P<0.05)。(2)CD44v5蛋白在食管鳞癌患者血清中的含量(31.308±10.123)μg/L高于健康体检者(19.364±1.680)μg/L,差异具有统计学意义(P<0.01);(3)ELISA法检测血清中CD44v5含量诊断食管鳞癌试验中,ROC曲线下面积0.865,以健康体检者血清CD44v5含量的95%可信区间上限值作为阳性判断标准诊断效果:灵敏度91.0%,特异度60.0%。结论:CD44v5的表达与食管鳞癌的发生发展及浸润转移有关;ELISA法检测血清中CD44v5蛋白含量用于诊断食管鳞癌灵敏度高,或许可以用于食管癌的筛检。Objective To detect the expression of CD44v5 in esophageal squamous carcinoma （ESCC） and analyze its relationship with clinical pathological features, so as to explore the value of detecting CD44v5 in serum for screening ESCC. Methods The CD44v5mRNA and protein in ESCC tissues and its corresponding adjacent non-carcinomatous tissues were detected by RT-PCR and Western-Blot. Enzyme-linked immunosorbent assay （ELISA） was applied to detect the protein in serum of 100 ESCC patients and 60 healthy subjects. Results （1）The expression of CD44v5 in esophageal squamous carcinoma tissues is higher than the tissue adjacent to carcinoma, and CD44v5 expression in different TNM stages, invasion depth, presence of lymph node metastasis and differentiation is with statistical differences（P 〈 0.05）. （2）CD44v5 proteins in patients with ESCC （31.308 ±10.123） μg/L is higher than healthy subjects （19.364 ± 1.680） μg/L, the difference is statistically significant （P 〈 0.01）. （3）In the trial of using ELISA method to detect CD44v5 content in serum in the diagnosis of ESCC, the area under the ROC curve is 0.865. If the healthy subjects＇ serum content of the upper limit of 95% confidence interval was taken as a positive judgment standard, the diagnosis effect： sensitivity is 91.0% and speciality is 60.0%. Conclusion The expression of CD44v5 was related to the occurrence and lymph node metastasis of ESCC. Using ELISA method to detect the contents of CD44v5 proteins in serum, with a high sensitivity, can be used to screen ESCC.

关 键 词：食管肿瘤 肿瘤 鳞状细胞 CD44V5 酶联免疫吸附测定 

分 类 号：R735.1[医药卫生—肿瘤]