液相色谱-串联质谱法测定人血浆中奥美拉唑的血药浓度及其药动学  

Determination of omeprazole concentration in human plasma by LC-MS/MS and pharmacokinetics study

作　　者：任秀华[1] 雷永芳[1] 陈倩[1] 付伟[1] 张冬林[1] 刘东[1] 杜光[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,湖北武汉430030

出　　处：《中国医院药学杂志》2015年第14期1282-1286,共5页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立液相色谱-串联质谱法测定人血浆中奥美拉唑的血药浓度并进行药动学研究。方法：以兰索拉唑为内标,血浆样品经乙腈沉淀后,经LC-MS/MS分离分析。采用Diamonsil C18柱（2.1 mm×150 mm,5μm）,甲醇-水（含0.01%甲酸）（67∶33）为流动相;流速为0.3 ml·min^-1,采用电喷雾离子源（ESI）,以多反应监测方式（MRM）进行负离子监测,奥美拉唑和兰索拉唑的定量分析离子对分别为m/z 344.2/193.8,367.9/163.8。结果：奥美拉唑在3.38～2 110.00ng·ml^-1（r=0.997 1）范围内线性关系良好,最低定量限为3.38 ng·ml^-1,方法回收率在92.48%～99.39%,日内（n=5）RE均小于3.96%,日间（n=15）RE均小于6.41%。结论：该方法快速简便,灵敏准确,可用于奥美拉唑血药浓度监测和药动学研究。OBJECTIVE To establish a high performance liquid chromatography tandem mass spectrometry（LC-MS/MS）method for determination of omeprazole（OM）in human plasma,and apply to clinical study on pharmacokinetics.METHODSPlasma samples were precipitated with acetonitrile and determined by LC-MS/MS using lansoprazole as an internal standard.Separation was carried out on a Diamonsil C18column（2.1 mm×150 mm,5μm）with a mobile phase of methanol-water（containing 0.01%formic acid）（67∶33）and at a flow rate of 0.3 ml·min^-1.ESI was performed in MRM negative mode using target ions at m/z344.2/193.8 for OM,and 367.9/163.8 for lansoprazole,respectively.RESULTS Calibration curves of OM were obtained in range of 3.38-2 110.00ng·ml^-1（r=0.997 1）with good linearity.The lower limit of quantization was 3.38ng·ml^-1.The relative recovery was 92.48%-99.39%,and intra-and inter-day RE less than 6.41%.CONCLUSION This method has good sensitivity and precision,and is suitable for plasma concentration monitoring and pharmacokinetic studies of OM.

关 键 词：奥美拉唑 液相色谱-串联质谱法 药动学 

分 类 号：R917.101[医药卫生—药物分析学]