人口腔鳞癌细胞系对淋巴靶向葫芦素BE聚乳酸纳米微粒的敏感性研究  

Effects of Cucurbitacin BE Polylactic Acid Nanoparticles on Human Oral Squamous Carcinoma Cell Lines

作　　者：杨凯[1] 王昌美[2] 温玉明 李龙江 王晓毅 陈绍维[3] 

机构地区：[1]重庆医科大学附属第一医院口腔颌面外科,400016 [2]四川大学华西口腔医学院 [3]中山大学附属第二医院口腔外科

出　　处：《华西口腔医学杂志》2002年第4期293-295,共3页West China Journal of Stomatology

基　　金：卫生部科学基金 (编号 98_1_2 2 1);重庆医科大学附属第一医院科研启动基金 (编号 2 0 0 2 0 0 7)

摘　　要：目的 :探讨人口腔鳞癌细胞系 (Tca8113和BcaCD885 )对颈淋巴结靶向性葫芦素BE聚乳酸纳米微粒 (CuBE_PLA_NP)的敏感性。方法 :用四唑盐显色法 (MTT法 )检测用不同剂量的CuBE_PLA_NP和CuBE在 1～ 8d内 8个时间点对Tca8113和BcaCD885的抗癌活性 ,计算出 2种药物在 8个时间点对 2种癌细胞的抑癌率和药物的半数抑癌率浓度。结果 :CuBE_PLA_NP和CuBE对Tca8113和BcaCD885均有强的杀伤作用 ,其杀伤效应均呈时间依赖性和剂量依赖性。结论 :经改型后的淋巴靶向CuBE_PLA_NP没有降低CuBE成份的抗癌活性 ,同时 ,由于CuBE_PLA_NP具有淋巴靶向性 ,更有利于杀灭口腔癌颈淋巴结转移灶内的癌细胞。Objective:The aim of this study was to study cytotoxic effects of cucurbitacin BE polylactic acid nanoparticles (CuBE_PLA_NP) on human oral squamous carcinoma cell lines Tca8113 (tongue carcinoma) and BcaCD885 (buccal carcinoma).Methods:The chemosensitivity of Tca8113 and BcaCD885 to CuBE_PLA_NP and CuBE was tested using MTT assay, the inhibitory rate and 50% inhibition concentrations values (IC_50) of these two drugs against these two cancer cell lines were evaluated in 1,2,3,4,5,6,7 and 8 days with different concentrations.Results:Both CuBE_PLA_NP and CuBE showed very high anti_cancer effects on Tca8113 and BcaCD855, and the anticancer efficacy of these two drugs was dose_ and time_dependent.Conclusion:The effects of CuBE_PLA_NP on lymph nodes of metastatic cancer cells were higher than that of CuBE.\;

关 键 词：敏感性研究 葫芦素BE 聚乳酸 钠米微粒 口腔癌 颈淋巴结转移 靶向化疗 四唑盐显色法 治疗 

分 类 号：R739.8[医药卫生—肿瘤]