金钗石斛总多酚提取工艺及体外抗氧化活性  

Extraction conditions and in vitro antioxidant activities of polyphenols from Dendrobium nobile

作　　者：费雯[1] 淳泽[2] 何沁嶷 王淼雨 罗傲雪[1] 范益军[1] 

机构地区：[1]四川农业大学风景园林学院,成都611130 [2]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2015年第4期623-628,共6页Chinese Journal of Applied and Environmental Biology

基　　金：四川省教育厅重点项目(12ZA274)资助~~

摘　　要：金钗石斛(Dendrobium nobile)具有多种保健作用,药用价值突出.通过Box-Behnken响应面分析法,研究金钗石斛总多酚的最佳提取工艺,并评估其体外抗氧化活性.采用溶剂法提取总多酚,在单因素实验基础上,利用BoxBehnke响应面分析法对p H值、乙醇浓度和料液比等参数进行优化,寻找最佳工艺.抗氧化活性以Vc为阳性对照,通过体外自由基清除实验评价金钗石斛总多酚的抗氧化活性.结果显示,当乙醇浓度为72%(V/V),料液比为1:51(g/m L),p H值为3时金钗石斛总多酚提取效率最高,为86.2%.体外抗氧化实验显示,金钗石斛总多酚对ABTS、DPPH自由基和羟基自由基活性均有不同程度的清除作用,其清除能力与总多酚质量浓度呈正相关,在浓度为5 mg/m L时对ABTS(Agreement on Basic Telecommunication Services)、DPPH(1,1-diphenyl-2-picrylhydrazyl)、羟自由基3种自由基的清除率分别为100%、78.54%和49.1%.本研究通过响应面法得到了提取得率及多酚含量较高的金钗石斛总多酚最优提取工艺条件,且得到的金钗石斛总多酚有良好的抗氧化能力.Dendrobium nobile has various health care functions and therefore of prominent medicinal value. This study aimed to find out optimal extraction conditions of polyphenols from Dendrobium nobile and to understand their antioxidant activity in vitro. With a single-factor experiment, the main parameters affecting the yield of phenolics were defined, including the pH of solvent, ethanol concentration and ratio of water to raw material. The antioxidant activities of the total polyphenols extract were evaluated by ABTS radicals, DPPH radicals and hydroxyl radicals using Vc as control. The optimal extraction parameters for total polyphenols yield were ethanol concentration 72% （V/V）, ratio of water to raw material as 1：50 （g/mL）, pH of 3, to obtain an extraction yield of total phenolic of 0.862. With the concentration of total phenolic from Dendrobium nobile as 5 mg/mL, the radical scavenging activity in clearing ABTS, DPPH and OH free radicals was 100%, 78.54% and 49.1%, respectively. The results suggested that response surface methodology is applicable for optimizing extraction conditions of total polyphenols from Dendrobium nobile, and that the total polyphenols from Dendrobium nobile have good scavenging activity in a close-dependent manner.

关 键 词：金钗石斛 多酚 响应面法 抗氧化 

分 类 号：R282.71[医药卫生—中药学]