酶法原位制备过氧乙酸对活性艳蓝KN-R氧化脱色的工艺  

Decolorization of remazol brilliant blue KN-R by the in-situ enzymatically generated peracetic acid

作　　者：武海龙[1] 舒正玉[1] 林红[1] 李婷[1] 刘艳如[1] 叶菲 慕向朵 李欣[1] 江贤章[1] 黄建忠[1] 

机构地区：[1]福建师范大学工业微生物发酵技术国家地方联合工程研究中心,福建师范大学教育部工业微生物工程中心,福建师范大学生命科学学院,福州350108

出　　处：《环境科学学报》2015年第8期2400-2406,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.31370802);福建省科技厅重点项目(No.2013H0021);福建省自然科学基金杰青项目(No.2009J06013)~~

摘　　要：利用过水解酶催化合成过氧乙酸,过氧乙酸原位氧化活性艳蓝KN-R脱色.在单因子试验的基础上,通过正交优化试验确定活性艳蓝KN-R的最佳脱色条件为:在5 m L反应体系中,最适p H 5.0,加酶量为20 U·反应-1,乙酸乙酯对过氧化氢的摩尔比率为40∶1和活性艳蓝KNR的浓度为80 mg·L-1.在此条件下反应6 h后,活性艳蓝KN-R的脱色率为81.11%,24 h后的脱色率为91.96%.在50倍放大试验中,该工艺24小时的脱色率为84.55%.A novel biological and chemical coupled treatment technology for decolorization of Remazol Brilliant Blue R was developed in this research work. Peracetic acid was first enzymatically prepared with perhydrolysis reaction and then applied to in situ oxidize Remazol brilliant blue KN-R（ RBBR）,one of the widely used dyes in the printing and dying industry. Based on the results of the single factor test,the optimal decolorization conditions for RBBR was then obtained via orthogonal experiment. The optimized physical and chemical factors for the oxidative decolorization of RBBR were found to be： 20 U per reaction enzyme dosage,the molar ratio of ethyl acetate to hydrogen peroxide at 40 ∶ 1,80 mg·L^- 1RBBR concentration,and intial p H5. 0 in the 5 m L decolorization system. The maximal decolorization of RBBR reached 81. 11% for 6 h and 91. 96% for 24 h in the 5 m L decolorization system,respectively. However,the maximal decolorization decreased to 84. 55% for 24 h in the 250 m L decolorization system.

关 键 词：过水解酶 过氧乙酸 原位制备 脱色 活性艳蓝KN-R 

分 类 号：X703[环境科学与工程—环境工程]