角膜修复用丝素蛋白膜的研制  

Development of Silk Fibroin Composite Films for Corneal Repair

作　　者：张珊珊[1] 李姣姣[1] 张芳[1] 张晓峰[2] 卢神州[1] 

机构地区：[1]苏州大学现代丝绸国家工程实验室,苏州215123 [2]苏州大学附属第一医院眼科,苏州215006

出　　处：《生物医学工程学杂志》2015年第4期867-873,886,共8页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(51373114);江苏省自然科学基金资助项目(BK20131176)

摘　　要：在角膜组织修复过程中,设计长时间保持透明的生物活性材料,是角膜修复材料研究的前沿问题。本文讨论了甲酰胺/丝素复合膜作为角膜基质修复材料的研究。将丝素蛋白和甲酰胺以不同比例共混后鼓风干燥获得透明丝素蛋白膜。甲酰胺/丝素复合膜(1∶10)具备优异的力学性能、细胞相容性和长时间保持透明的特性。将兔角膜基质细胞接种于复合膜5h后,细胞黏附率达90%以上,复合膜与培养板上的细胞增殖率没有显著差异。甲酰胺/丝素复合膜作为角膜基质修复材料值得进一步探讨研究。It is important to design a long-period transparent bioactive material for corneal repair in the process of corneal tissue renovation. This article discusses the silk fibroin and formamide blend membranes as a corneal stroma repair material. Silk fibroin solution was mixed with formamide in different proportions to obtain insoluble transparent silk fibroin film by casting method. The blending membranes had excellent mechanical properties, cell compatibility and long-term transparent properties. Rabbit corneal stromal cells were seeded on the sterilized composite films. The rate of cell surface adhesion was over 90% after cells were placed on it for 5 hours. When cells were seeded on blend membranes from one day to seven days, Alma Blue was added to complete medium. Compared with the cell culture plate, there was no significant difference in cell proliferation on formamide/silk films. The results indicated that formamide/silk films might be used as a corneal stroma repair material and worth of further investigation.

关 键 词：丝素蛋白 角膜 复合膜 透光率 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]