石决明水提液对白内障大鼠晶状体上皮细胞内质网应激影响的研究  

Effects of the water extract of the drug Shijueming on endoplasmic reticulum stress in lens epithelial cells of rats with D-galactose cataract

作　　者：王玉斌[1] 金鑫[2] 徐清妍 李秀娟[1] 祁磊[1] 郑玛丽[1] 钟瑞生 林媛[1] 

机构地区：[1]厦门市中医院眼科,福建厦门361009 [2]厦门大学医学院药学系,361102

出　　处：《中国实用眼科杂志》2015年第8期943-946,共4页Chinese Journal of Practical Ophthalmology

基　　金：2012年福建省卫生厅中医药科研专项课题（WST201210）;2013年福建省卫生厅中医药科研专项课题（wzhw201302）;2014年厦门市科技局科技惠民项目课题（3502220144030）

摘　　要：目的探究石决明水提液对白内障大鼠晶状体的保护作用机制。方法实验研究。于2012年12月至2013年12月在温州医科大学药学院实验室，将100只大鼠按体重编号采用随机数字表随机分为4组，每组25只，制作D-半乳糖性白内障大鼠模型后，空白组和模型组分别点生理盐水，白内停组给白内停滴眼液滴眼，石决明组给石决明水提液。造模成功后处死大鼠，取出晶状体，免疫组化测定CHOP表达，Western Blot方法检测GRP78的表达，荧光定量PCR测定XBP—1mRNA表达。结果石决明组和白内停组CHOP，GRP78，XBP—1mRNA的表达均低于模型组，有显著性差异（P〈0．01），石决明组CHOP和GRP78的表达与白内停组无显著性差异（P〉0．05），石决明组XBP—1mRNA的表达低于白内停组，有显著性差异（P〈0．01）。结论石决明水提液对白内障大鼠的晶状体有保护作用，作用等同或者优于白内停。Objective To investigate the protect mechanism of Shijueming on the rats with D-galactose cataract. Methods One hundred healthy SD rats were randomly divided into four groups （blank group, control group, Baineiting eyedrops group, Shijueming eyedrops group）, D-galactose cataract models were established. Blank and control group were treated with physiological saline eye- drops. Baineiting eyedrops group was treated with Baineiting eyedrops. Shijueming eyedrops group was treated with 0.25% Shijueming eyedrops （three times each day, one drop each time）. The levels of CHOP, GRP78 and XBP-1mRNA were measured by immunohistochemistry, Western blot and RT-PCR methods. Results The expression of CHOP, GRP78 and XBP-1mRNA in shijueming eye- drops group were lower than in control group （P 〈0.05）. Conclusions The water extract of Shijueming can decrease endoplasmic reticulum stress in lens epithelial cells of rats with D-galactose cataract and protect lens.

关 键 词：石决明 白内障 内质网应激 

分 类 号：R776.101[医药卫生—眼科]