缺血性脑卒中患者血清中miR-145、miR-497表达变化及其与Hs-CRP、MMP-9的相关性  

Changes of serum miR-145 and miR-497 levels and their correlations with high sensitive C- reactive protein and matrix metalloproteinase-9 in patients with ischemic stroke

作　　者：罗振钊[1] 孔曼[1] 卢忠心[1] 王静[1] 

机构地区：[1]华中科技大学同济医学院附属武汉市中心医院检验科,武汉430014

出　　处：《中华神经医学杂志》2015年第8期770-774,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金面上项目（31171029）;武汉市中心医院博士基金（YB13A06、YB15804）

摘　　要：目的探讨miR-145、miR．497在缺血性脑卒中患者血清中的表达水平及其表达变化和超敏C反应蛋白（Hs-CRP）、基质金属蛋白酶-9（MMP-91的相关性。方法选择武汉市中心医院自2013年1月至10月收治的126例缺血性脑卒中患者（患者组，包括轻度神经功能缺损28例、中度神经功能缺损61例、重度神经功能缺损37例）和同期107例健康体检者（对照组）为研究对象，采用荧光定量PCR法检测血清中miR．145、miR-497的表达水平．采用免疫比浊法及ELISA法分别检测Hs-CRP和MMP-9的表达水平，采用偏相关分析法探索患者组血清中miR-145、miR-497的表达变化与Hs-CRP、MMP-9的相关性。结果（1）患者组血清中miR-145、miR-497及Hs-CRP、MMP-9的表达水平明显高于对照组，差异均有统计学意义（氏0．05）。偏相关分析法结果显示：患者组血清中miR-145的表达水平与miR-497、CRP、MMP-9都存在正相关性（r=0．718，P=-0．000；r=0．658，P=-0．000；r=0．455，P=0．000），miR-497的表达水平与CRP、MMP-9都存在正相关性（r=0．845，P=0．000；r=0．787，P=0．000）。（2）重度神经功能缺损患者血清中miR-145、miR-497及Hs—CRP、MMP-9的表达水平明显高于中度、轻度患者，中度神经功能缺损患者血清中miR-145、miR-497及Hs=CRP、MMP=9的表达水平明显高于轻度患者，差异均有统计学意义（P〈0．05）；并且在不同程度神经功能缺损患者血清中，miR-145、miR=497和Hs=CRP、MMP-9的表达水平之间具有不同的相关强度。结论患者血清中miR=145、miR-497可能可以作为诊断缺血性脑卒中的分子标志物，并且二者在缺血性脑卒中的发生发展过程中可能具有协同调控作用。Objective To explore the changes of serum miR-145 and miR-497 levels and their correlations with high sensitive C-reactive protein （Hs-CRP） and matrix metalloproteinase-9 （MMP-9） in patients with ischemic stroke. Methods One hundred and twenty-six patients with ischemic stroke, including 28 mild neurologic impairment （NI） patients, 61 moderate NI patients and 37 severe NI patients, and 107 normal controls, were collected in our hospital from January 2013 to October 2013. Expressions of serum miR-145 and miR-497 were measured by real-time fluorescent quantitative PCR, protein markers Hs-CRP and MMP-9 were determined by immune turbidimetry and ELISA assay, respectively, correlations between microRNAs （miRNAs） and protein markers were analyzied by partial correlation analysis. Results Both expressions of serum miR-145 and miR-497, and Hs-CRP and MMP-9 levels in ischemic stroke patients were significantly elevated as compared with those in the normal controls （P〈0.05）. Positive correlations were noted between of serum miR-145 expression and both serum miR-497 expression and expressions of serum Hs-CRP and MMP-9 （r=0.718, P=0.000; r= 0.658, P=0.000; r=0.455, P=0.000）; meanwhile, serum miR-497 expression and expressions of serumHs-CRP and MMP-9 were also positively correlated （r=0.845, P=0.000; r=0.787, P=0.000）. Severe NI patients had significantly higher expressions of serum miR-145 and miR-497, and Hs-CRP and MMP-9 levels as compared with mild NI patients and moderate NI patients （P〈0.05）, and these levels in the moderate NI patients were significantly higher than those in the mild NI patients （P〈 0.05）; these miRNAs and protein markers showed a different correlation strength depended on the different severities of neurologic impairment. Conclusion Both of serum miR-145 and miR-497 levels may be useful biomarkers for screening of ischemic stroke; and miR-145 and miR-497 may have synergistic effect on regulating the pathogenesis of ischemic stroke.

关 键 词：缺血性脑卒中 MIR-145 miR-497 超敏C反应蛋白 基质金属蛋白酶-9 

分 类 号：R743.3[医药卫生—神经病学与精神病学]