苦味酸-天狼猩红染色法检测PHEMA人工角膜植入碱烧伤兔角膜中胶原的动态变化  

Observation of collagen in PHEMA Keratoprothesis implanted into alkali burned rabbit corneas by picric sirius red staining

作　　者：白华[1,2] 郭惠玲[3] 王丽强[2] 黄一飞[2] 

机构地区：[1]北京军区总医院眼科,北京100700 [2]解放军总医院眼科,北京100853 [3]中国人民解放军第306医院眼科,北京100853

出　　处：《山东大学耳鼻喉眼学报》2015年第4期69-73,共5页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：国家重点基础研究发展规划项目(973计划)(2013CB967001);国家自然科学基金(81271052);国家自然科学基金(81170830);国家自然科学基金(30973245);国家自然科学基金(31271059);解放军总医院临床扶持基金(2012fc-tsys-1003)

摘　　要：目的观察新型软性一体式PHEMA人工角膜对碱烧伤兔角膜孔隙内新生组织中胶原成分及分布的影响。方法制备兔角膜碱烧伤模型后,植入软性一体式PHEMA人工角膜材料。分别于术后2、8、16、22周取裙边组织进行苦味酸-天狼猩红染色,偏振光下进行观察。结果这种PHEMA材料中允许角膜及周围组织迁入并生长,多部位形成纤维连接。染色后偏振光下提示孔隙内新生组织主要为Ⅰ型胶原,其生长过程表现为黄绿色的Ⅲ型胶原慢慢变成成熟的、红色的Ⅰ型胶原,并且角膜缘处细胞活跃。结论 PHEMA材料孔隙的胶原和角膜组织的胶原变化与植入正常兔角膜的愈合结果一致。Objective To observe the dynamic changes of type and distribution of collagen fiber in the porous skirt of newtype one-piece keratoprosthesis（ KPros） made of PHEMA implanted into the alkali burned rabbit corneas.Methods A total of 10 pieces KPros were implanted into the alkali burned rabbit corneas. The skirt of kpro was obtained after 2,8,16 and 22 weeks. The samples were treated with picric-sirius red staining and observed by polarizing microscopy. Results This material PHEMA allowed the cornea and surrounding tissue growing and forming a multi-site stable fiber-connections under polarized light after staining. The neworganization within the pores was mainly type I collagen and its growth performance was that yellow-green type Ⅲ collagen become mature red typeⅠ collagen in process and cells were active especially in limbus. Conclusion The dynamic changes of collagen observation,can be concluded that the PHEMA pores and the cornea shown the similar performance and consistent with normal rabbit corneal implant healing.

关 键 词：人工角膜 碱烧伤 聚甲基丙烯酸羟乙酯 苦味酸-天狼猩红 偏振光显微镜 

分 类 号：R779.65[医药卫生—眼科]