胞嘧啶脱氨酶和尿嘧啶磷酸核糖转移酶融合基因对食管癌裸鼠移植瘤的抑制作用  

Cytosine deaminase and uracil phosphoribosyl transferase inhibition of transplantation tumor gene fusion

作　　者：潘雪[1] 李向楠[2] 王鹏[1] 侯晓旭[3] 

机构地区：[1]郑州大学护理学院,450052 [2]郑州大学第一附属医院胸外科 [3]郑州大学第一附属医院麻醉科

出　　处：《中华实验外科杂志》2015年第8期1823-1825,共3页Chinese Journal of Experimental Surgery

基　　金：河南省教育厅自然科学基础研究计划资助项目（13A320415）;郑州市科技局科技攻关计划资助项目（083SGYS33262）

摘　　要：目的观察多药耐药基因1（MDRl）启动子调控的胞嘧啶脱氨酶和尿嘧啶磷酸核糖转移酶融合基因（CD：UPRT）在裸鼠体内对食管癌细胞EC9706细胞的杀伤作用。方法0．2mlEC9706细胞悬液接种裸鼠，构建食管癌皮下移植瘤模型及pAdTrack—MDRl-CD：UPRT质粒，模型随机分成3组经处理并观察30d，测量肿瘤体积使用V=XXy2／2（x为长径，y为短径）。肿瘤组织常规病理检查，免疫组织化学检测CD蛋白的表达。结果成功构建食管癌皮下移植瘤模型，处理30d后，B、C组肿瘤生长迅速，表面出现坏死，肿瘤体积分别为（3．21±0．23）、（3．15±0．22）cm3；A组肿瘤生长受到抑制，第30天体积为（0．98±0．15）cm3，差异有统计学意义（P〈0．05）。A组肿瘤细胞排列稀疏，大片坏死红染，部分可见胞核固缩、核碎裂；B、C组肿瘤细胞排列紧密，血管丰富，细胞形态及细胞核呈多形性，核大深染，核分裂像多见。经免疫组织化学染色后，A、B组肿瘤组织呈阳性反应，表明有CD蛋白的表达，C组肿瘤组织免疫组织化学染色呈阴性。结论MDR1启动子调控的CD：UPRT／5-氟胞嘧啶（5-FC）融合自杀基因系统对裸鼠食管癌皮下移植瘤有显著的杀伤作用。Objective To study the multidrug resistance gene 1 （ MDR1 ） promoter regulated cyto- sine deaminase and uracil phosphoribosyltransferase fusion gene （CD： UPRT） killing of esophageal cancer cells in nude mice EC9706 cells. Methods 0. 2 ml EC9706 cells were inoculated subcutaneously in nude mice and esophageal cancer xenograft model to build and build pAdTrack - MDR1 - CD ： UPRT plasmids, were randomly divided into three groups of treated and observed 30 d, tumor volume was measured using the V = x x y2/2 （x long diameter, y for short diameter）. Routine pathological examination of tumor tis- sue, immunohistochemical detection of CD protein. Results Successfully constructed subcutaneous xeno- graft model of esophageal cancer, treatment for 30 days, group B, group C rapid tumor growth, surface necrosis, tumor volume was （3.21 ±0. 23） cm3 , （3.15 ±0.22） cm3 ; A group of tumor growth restrained, the first 30 days volume （cm3）, compared to the difference of （0. 98 ＋ O. 15 ） was statistically signifi- cant （P 〈0. 05）. A group of tumor cells arranged in a sparse, large areas of necrosis red dye, partially visi- ble condensation nuclei, nuclear fragmentation; group B, group C tumor cells tightly packed, rich in blood vessels, cell morphology and cell nuclei were pleomorphic, and a large stained mitotic common. After immu- nohistochemieal staining, A, group B tumor tissues were positive, indicating that the protein expression of CD, group C immunohistochemical staining of tumor tissue was negative. Conclusion MDR1 promoter regu- lated CD ： UPRT/5 - fluorocytosine （5 - FC） fusion suicide gene system for esophageal carcinoma in nude mice xenograft significant cytotoxicity, gene therapy for esophageal cancer provides a possible approach.

关 键 词：食管癌 胞嘧啶脱氨酶 尿嘧啶磷酸核糖转移酶 5-氟胞嘧啶 

分 类 号：R735.205[医药卫生—肿瘤]