核转录因子-kB信号通路对甲状腺乳头状癌细胞生物学行为的影响  

Effects of nuclear factor - kB signaling on the biological behavior of papillary thyroid carcinoma

作　　者：樊玉霞[1] 刘洋[1] 刘征[1] 王晓明[1] 袁青领[1] 卢秀波[1] 

机构地区：[1]郑州大学第一附属医院甲状腺外科,450052

出　　处：《中华实验外科杂志》2015年第8期1866-1868,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年基金资助项目（81202112）

摘　　要：目的观察核转录因子-kB（NF-kB）信号通路对甲状腺乳头状癌（PTC）细胞株CGTHW3乍物学行为的影响。方法培养PTC细胞株CGTHW3，将细胞随机分为3组：NF-kB信号通路激活剂组、NF-kB信号通路抑制剂组及空白对照组。用细胞计数试剂盒（CCK-8）法观察细胞增殖情况，流式细胞仪分析细胞凋亡率及周期分布，Transwell小室实验评价迁移能力。结果（1）NF-kB信号通路的激活可显著促进CGTHW3细胞的增殖；（2）与NF-KB信号通路抑制剂组[（59．17±2．32）％]和空白对照组[（28．50±1．71）％]比较，NF-kB信号通路激活剂组的细胞凋亡比例[（19．85±0．93）％]明显下降（P〈0．05）；（3）与NF-kB信号通路抑制剂组和空白对照组比较，NF-kB信号通路激活剂组G。期细胞显著降低，而s期细胞显著升高（P〈0．05）；（4）NF-kB信号通路激活组细胞迁移数为（157±8）个，与NF-kB信号通路抑制剂组[（30±2）个]和空白对照组[（52±4）个]比较，细胞迁移能力明显增强（P〈0．05）。结论NF。KB在PTC细胞增殖、凋亡及迁移等过程中起重要作用。Objective To observe the effects of nuclear factor -kB （ NF -kB） signaling on the biological behavior of papillary thyroid carcinoma cell line CGTH W3. Methods Cultured CGTIt W3 ceils were randomly divided into 3 groups： NF -kB signaling activator lipopolysaccharide （LPS）, NF - kB signa- llng inhibitor PDTC and phosphate buffer （PBS）. Cell proliferation was observed with （;ell counting kit -8 （CCK- 8） method. Cell apoptosis rate and cell cycle distribution were analyzed by flow cytometry. The migration ability was tested by transwell methods. Results （ 1 ） Activation of NF - kB signaling couht promote the proliferation of CGTH W3 cell line. （2） Compared with group of NF - KB signaling inhibitor （ 59. 17 ± 2. 32） % and blank control group （ 28.50 ± 1.71 ） %, the cell apoptosis ration of NF - KB signa- ling activator group （ 19.85 ± 0. 93） % decreased significantly （ P 〈 0. 05 ）. （ 3 ） Compared with group of NF-KB signaling inhibitor and blank control group, the G1 phase was dramatically reduced and the S phase was significant increased （ P 〈 0.05 ）. （4） Compared with group of NF - kB signaling inhibitor （30 ± 2） and blank control group （52 ± 4）, the cell migration number of NF -kB signaling activator group was 157 ± 8 （ P 〈 0. 05）. Conclusion NF - kB signaling pathway plays an important role in the promo- tion of （;ell proliferation and migration ability, inhibition of ceil apoptosis of PTC cell line CGTH W3, which establishs the NF -kB signaling pathway as a target for tumor therapy.

关 键 词：甲状腺乳头状癌 核转录因子-kB信号通路 增殖 凋亡 迁移 

分 类 号：R735.37[医药卫生—肿瘤]