微小RNA-9对胃癌细胞SGC-7901增殖及凋亡的影响  

Effect of microRNA - 9 on the proliferation and apoptosis of gastric cancer cell line SGC - 7901

作　　者：王公平[1] 周博[1] 金灿辉[1] 汪增方 杨言通[1] 陈晔[1] 张伟[1] 王珍珍[1] 裴哲[1] 冯笑山[1] 

机构地区：[1]河南科技大学第一附属医院肿瘤外科,洛阳471003

出　　处：《中华实验外科杂志》2015年第8期1899-1901,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA（miRNA，miR）-9对胃癌细胞增殖及凋亡的影响，以及对B细胞淋巴瘤／白血病-2（bcl-2）及bcl-2相关X蛋白（bax）蛋白表达的调控作用。方法将50nmol/L的miR-9mimics及阴性对照序列由Lipofectamine2000转染入人胃癌SGC-7901细胞中；实时定量反转录聚合酶链反应（RT-qPCR）法检测转染后胃癌细胞内miR-9表达水平；细胞计数试剂盒（CCK．8）法检测细胞增殖能力；划痕实验检测细胞迁移能力；流式细胞术分析转染后细胞凋亡的变化；Westernblot法检测bcl-2、bax蛋白表达水平。结果转染24h，miR-9mimics转染组细胞内miR-9表达水平较空白对照组明显升高，约为空白对照组的（45．69±2．67）倍，差异有统计学意义（F=179．45，P〈0．05）；与空白对照组比较，miR-9mimics转染组细胞增殖受抑制（P〈0．05），24、48、72h细胞增殖抑制率分别为18．45％、35．12％、53．78％，凋亡细胞增多（P〈0．05），bcl-2蛋白表达下调，bax表达上调。结论miR-9能够抑制SGC7901细胞增殖并诱导胃癌细胞凋亡，这可能与其抑制bcl-2蛋白的表达有关。Objective To investigate the effect of microRNA （miR） -9 on proliferation and ap- optosis of gastric cancer ceils, and on the regulation of expression of bcl - 2 and bax protein. Methods MiR - 9 mimics and 50 nmol/L negative control sequence is composed of 2000/L Lipofectamine was trans- fected into human gastric cancer SGC -7901 cells; real -time reverse transcriptase -polymerase chain re- action （RT -qPCR） was detected after transfection in gastric cancer ceils miR -9 expression level; ability of cell counting kit - 8 （ CCK - 8 ） method to detect cell proliferation; changes of apoptosis after transfection were analyzed by flow cytometry; detection of bcl - 2 Western blotting method, the expression of bax protein the level of. Results 24 cells were transfected with H, miR - 9 mimics expression level of miR - 26 in cells transfected group compared with blank control group increased significantly （ P 〈 0. 05 ） ; Compared with the blank control group, cell proliferation was inhibited in miR - 26mimics transfection group （ P 〈 0. 05 ）, the apoptotic cells increased （P 〈 0. 05 ）, bcl - 2 protein expression down regulated, the up regulation of bax expression. Conclusion MiR -26 can inhibit the proliferation of SGC7901 cells and induce apoptosis of gastric cancer cells, the expression of which may be related to the inhibition of bel - 2 protein.

关 键 词：微小RNA-9 胃癌 SGC-7901 增殖 凋亡 

分 类 号：R364.4[医药卫生—病理学]