肝再生磷酸酶-3促进结肠癌细胞分泌趋化因子26对肿瘤相关性巨噬细胞的趋化作用  

The migration of tumor - associated macrophages was enhanced by phosphatase of regenerating liver- 3 through promoting human colon cancer cells to secrete chemokine 26

作　　者：黄伟华[1] 来伟[2] 蓝球生 曾献清 张旸[2] 褚忠华[2] 

机构地区：[1]广东省梅州市五华县人民医院外二科,514400 [2]中山大学孙逸仙纪念医院胃肠外科

出　　处：《中华实验外科杂志》2015年第8期1908-1910,共3页Chinese Journal of Experimental Surgery

基　　金：广东省科技计划国际合作项目（20128050600叭4）;梅州I“科技计划资助项目”（20148142）

摘　　要：目的观察肝冉生磷酸酶-3（PRL-3）促进结肠癌细胞LoVo分泌趋化I凡j予26（CCl26），对肿瘤相关性巨噬细胞的趋化、聚集作用。方法实时定量反转录聚合酶链反应（RT-qPCR）检测CCL26mRNA水平表达差异；酶联免疫吸附试验（ELISA）检测CCL26蛋白水平表达謦砰，检测添加核因子kB（NF—kB）抑制剂BAY11-7082（5、10、15moL／L）后，CCL26蛋白水平；Transwell旺移实验检测LoVo—P、LoVo—C与TAM共培养24h后，对TAM迁移作用。结果RT—qPCR检测结果显，Ji，LoVo—P对比LoVo—C，CCL26升高（46±5）倍，ELISA检测CCL26蛋白水平，LoVo—P为（91±5）ng／L，LoVo—C为（61±3）ng／L，LoVo．P加人核因子（NF）-KB抑制剂BAY11—7082（5、10、15mol／L）后，CCL26蛋Fj水平呈浓度梯度降低（P〈0．01）；Transwell迁移实验结果显示，LoVo—P对比LoVo—C明显对TAM有趋化作用，加入NF-kB抑制剂BAY 11-7082后，迁移作用明艟减弱，不同浓度的CCL26（1、10、100μg／L），能对TAM的趋化作用呈浓度梯度增高（P〈0．01）。结论PRL-3能促进钻肠癌LoVo细胞分泌CCL26，通过CC126可以诱导TAM趋化、聚集。Objective To investigate the phosphatase of regenerating liver - 3 （ PRL - 3 ） promo- ting colon cancer cells LoVo to secrete chemokine 26 （CCL26） , which enhances tumor - associated macro- phages （TAMs） migration. Methods We used real -time quantitative reverse transcriptase- polymerase chain reaction （RT- qPCR）, and enzyme linked immunosorbent assay （ELISA） to detect the mRNA and protein expression of CCL26, and the protein expression of CCL26 after treatment of LoVo eells with nucle- ar lactor-kB （NF- kB） inhibitor, BAY 11 -7082 （5, 10, and 15 tool/L）. Invasion assays were applied to determine the effect of CCL26 on the ability of PRL - 3 promoting migration of TAMs. Results RT - qPCR showed that the CCL26 mRNA expression of LoVo - P was higher than LoVo - C [ （ 46 ± 5 ） h）hl]. EL1SA displayed that the protein level of CCL26 of LoVo - P was higher than LoVo - C, and it was altcnuated in a concentration - dependent manner by treating LoVo - P with the NF - KB inhibitor, BAY 11 -7082 （5, 10, and 15 mol/L）. Transwell invasion assays showed that the migration of TAMs was enhamced when TAMs were cocultured with LoVo- P cells, and it can be inhibited by NF- KB inhibitor, BAY 11 -7082. Also the migration of TAMs was enhanced in a concentration -dependent manner when TAMs were cocuhured with different concentrations of CCL26 （ P 〈 0. 01 ）. Conclusion PRL - 3 promotes colon cancer cells to secrete chemokine CCL26, which can enhance TAMs migration.

关 键 词：肝再生磷酸酶- 结肠癌 肿瘤相关性巨噬细胞 迁移 

分 类 号：R73-37[医药卫生—肿瘤]