淫羊藿素对3种人肿瘤细胞增殖抑制作用的比较  

Inhibitory effects of icaritin on proliferation of, three human carcinoma cell lines

作　　者：张淑琴[1] 贠向阳 郑倩[1] 杨广笑[1] 何光源[1] 陈明洁[1] 

机构地区：[1]武汉市六医院暨江汉大学附属医院,430015 [2]科技部国际科技(基因工程)合作基地华中科技大学生命科学与技术学院

出　　处：《中华实验外科杂志》2015年第8期1919-1921,共3页Chinese Journal of Experimental Surgery

基　　金：武汉市科技攻关计划资助项目（201260523185）;国家自然科学基金生物理科基地学生能力提高项目（NSFC-J1103514）

摘　　要：目的比较淫羊藿素对人肝癌细胞HepG2、人肺癌细胞A549、人肾癌细胞786-O等3种人肿瘤细胞增殖的抑制效果，并探讨其作用机制。方法通过噻唑蓝（MTF）法比较淫羊藿素对肿瘤细胞HepG2、A549、786．0增殖的影响，流式细胞仪检测并确定淫羊藿素对肿瘤细胞凋亡的影响。Westernblot法检测淫羊藿素处理前后细胞增殖和凋亡相关蛋白合成的变化。结果淫羊藿素均呈时间和浓度依赖性地抑制3种人肿瘤细胞的增殖，其中对786-O的抑制效果最为明显，5μmol／L淫羊藿素处理24h细胞增殖抑制率达44％，半数抑制剂量（IC50）为5．85μmol／L；淫羊藿素促进3种肿瘤细胞凋亡，诱导786-O细胞凋亡的效果最显著，10μmol／L淫羊藿素处理24h后，凋亡细胞比率达到45．8％；抑制周期相关蛋白细胞周期蛋白（Cychn）A、CyclinD1、CyclinE、周期蛋白依赖性激酶2（CDK2）和B细胞淋巴瘤／白血病-2（bcl-2）的表达，提高促凋亡蛋白bcl-2相关X蛋白（bax）的表达。结论淫羊藿素在体外能明显抑制3种人肿瘤细胞的增殖，诱导其凋亡，对786-O效果最为显著；它可能通过调节细胞增殖和凋亡相关蛋白的表达。Objective To compare the effects of Icaritin on proliferation of human hepatocellular carcinoma cell line HepG2, human lung carcinoma cell line A549 and human renal cell carcinoma cell line 786 - O, and study the mechanism during those processes. Methods Methyl thiazol tetrazolium （MTT） assay was used to test the effect of Icaritin on proliferation of HepG2, A549 and 786 - O cells. Apoptosis of the cells was detected by flow cytometry. The expression of several key proliferative and apoptotic related proteins was investigated by Western blotting. Results Icaritin inhibited the proliferation of HepG2, A549 and 786 - O cells in a time - and dose - dependent way. The inhibition effect on 786 - 0 cells was the high- est with 44% inhibition rate after 5 μmol/L Icaritin treatment for 24 h and 50% inhibitory dose （ ICs0 ） was 5. 85μmol/L. Icaritin induced apoptosis in all the three cell lines with the highest effect on 786 - O cells with the apoptosis rate being 45. 8%. Icaritin reduced the expression of Cyclin A, Cyclin D1, Cyclin E, cyclin dependent kinase 2 （CDK2） and B cell lymphoma/leukemia - 2 （ bcl - 2）. The up - regulated pro- tein expression of bcl - 2 associated X protein （bax） was also observed. Conclusion Icaritin inhibited proliferation of HepG2, A549 and 786 - 0 cells and induced apoptosis significantly. The effect on 786 - O cells was the highest. This process may be involved in the regulation of several Cyclins, bcl - 2 - associated X protein （bax） and bcl-2.

关 键 词：淫羊藿素 肿瘤细胞 增殖 

分 类 号：R739.5[医药卫生—肿瘤]