动物性食品中氨苯砜dcELISA的检测  

Development of a direct competitive enzyme-linked immunosorbent assay for detection of dapsone in animal foods

作　　者：郑小娇[1] 张晶[1] 张亚卓[1] 史晨杉 王向红[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001

出　　处：《食品与发酵工业》2015年第7期189-192,共4页Food and Fermentation Industries

基　　金：河北省科技支撑项目(No.152776120D)

摘　　要：建立了动物性食品中氨苯砜残留快速检测技术。将氨苯砜重氮化后连接到牛血清蛋白（BSA）上制得免疫原BSA-DDS，免疫新西兰大耳白兔获得多克隆抗体，经ProteinA-Sephros 4B对抗体进行纯化，在此基础上建立直接竞争ELISA方法。结果显示：该方法可制备目标抗原BSA-DDS和抗体，氨苯砜与载体蛋白偶联比可达到1：10；建立的直接竞争ELISA方法的IC50为4．78ng／mL，最低检测限达0．03ng／mL，加标回收率为75．45％～91．75％。A direct competitive enzyme-linked immunosorbent assay （ELISA） was developed to detect Dapsone in animal foods. Hapten dapsone was conjugated with Bovine Sera Albumin （BSA） by diazotization to produce immunogen BSA-DDS used for immunizing rabbit. The rabbit antiserum was purified with ProteinA-Sephros 4B to prepare polyclonal antibody against dapsone. A direct enzyme-linked immunosorbent assay （ELISA） was developed to detect and quantitate dapsone. The bewt synthesis of BSA-DDS and the conjugated-ratio was 1 ： 10. The IC50 was 4.78 ng/mL, visual detection limit was 0.03 ng/mL,and recoveries of dapsone in spiked samples ranged from 75.45% - 91.75%. The artificial antigens can be used for preparing specificity anti-dapsone antibody. The method is sensitive and the procedure of sample pretreatment is simple and quick. It is suitable to use in the detection of DDS residues in animal foods on site.

关 键 词：氨苯砜 多克隆抗体 ELISA 

分 类 号：TS207.3[轻工技术与工程—食品科学]