肾透明细胞癌患者血清miR-362的异常表达及意义  

Abnormal expression of serum miR-362 in patients with clear-cell renal cell carcinoma and its clinical significance

作　　者：路美玲[1,2] 王成[2] 胡继成[3] 陈熹[4] 张辰宇[4] 张春妮[2] 殷志敏[1] 

机构地区：[1]南京师范大学生命科学院,南京210046 [2]南京军区南京总医院检验科,南京210002 [3]哈尔滨医科大学附属第五医院泌尿科,黑龙江大庆163000 [4]南京大学生命科学学院,医药生物技术国家重点实验室,南京210093

出　　处：《临床检验杂志》2015年第6期417-419,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81472021);南京军区医学科技创新重点课题(12Z28);南京军区南京总医院科研课题(2014043)

摘　　要：目的检测肾透明细胞癌患者血清miR-362水平,探讨其作为肾透明细胞癌分子诊断指标的可能性。方法采集107例肾透明细胞癌患者治疗前血清（其中TNMⅠ期76例、Ⅱ期16例、Ⅲ期2例、Ⅳ期8例及未知者5例）,用实时荧光定量RTPCR法检测各组miR-362含量,并以107例年龄、性别匹配的健康人作为对照组。结果 miR-362在肾透明细胞癌患者血清中的相对含量为0.81（0.65,1.15）,与健康对照组0.62（0.46,0.78）相比,表达水平明显升高（U=3 122,P〈0.01）。Ⅰ期、Ⅱ期和Ⅳ期患者血清miR-362的相对含量分别为0.76（0.66,0.95）、0.76（0.66,0.95）和0.69（0.37,1.03）,均明显高于健康对照组,差异有统计学意义（U分别为2 197、474和98,P均〈0.01）。miR-362诊断肾透明细胞癌的ROC曲线下面积（AUCROC）为0.727（95%CI：0.661~0.793）。当cut off值为0.696时,miR-362诊断肾透明细胞癌的敏感性和特异性分别为64.5%和63.6%。进一步对TNMⅠ期患者进行分析,结果其AUCROC为0.715（95%CI：0.641~0.790）。当cut off值为0.696时,miR-362诊断TNMⅠ期患者的敏感性和特异性分别为65.3%和63.6%。结论早期肾透明细胞癌患者血清中miR-362水平升高,具有一定的早期诊断潜能。Objective To investigate the levels of serum miR-362 in the patients with clear-cell renal cell carcinoma（ cc-RCC）,and evaluate its potential as a molecular biomarker for the diagnosis of cc-RCC. Methods The serum samples from 107 patients with untreated cc-RCC,including 76 TNM stageⅠ,16 stage Ⅱ,2 stage Ⅲ,8 stage Ⅳ and 5 unknown stage,and 107 healthy controls with matched age and sex,were collected to detect the levels of miR-362 by real-time quantitative RT-PCR（ qRT-PCR）. Results The median levels[M（ P25,P75） ]of serum miR-362 in the patients with cc-RCC were significantly higher than that in the controls[0. 81（ 0. 65,1. 15） vs0. 62（ 0. 46,0. 78）,U = 3 122,P〈0. 01]. The relative level of miR-362 in serum from patients with only TNM stageⅠ,Ⅱ or Ⅳ was0. 76（ 0. 66,0. 95）,0. 76（ 0. 66,0. 95） and 0. 69（ 0. 37,1. 03）,respectively,all being statistically markedly higher than controls [U was 2 197,474 and 98,respectively,P〈0. 01]. The area under the ROC curve（ AUCROC） of miR-362 for the diagnosis of cc-RCC was0. 727（ 95% CI： 0. 661-0. 793）. When the cut off value was 0. 696,the sensitivity and specificity of miR-362 for the diagnosis of cc-RCC were 64. 5% and 63. 6%. Furthermore,AUC for TNM stageⅠ was 0. 715（ 95% CI： 0. 641-0. 790）. When the cut off value was 0. 696,the sensitivity and specificity of miR-362 for diagnosing TNM stageⅠ was 65. 3% and 63. 6%,respectively. Conclusion The increased serum miR-362 levels in cc-RCC patients indicate that miR-362 may be helpful for the diagnosis of cc-RCC.

关 键 词：肾透明细胞癌 微小核糖核酸 miR-362 定量RT-PCR 早期诊断 

分 类 号：R781.64[医药卫生—口腔医学]