MYB蛋白表达在腺样囊性癌诊断中的意义及MYB基因拷贝数状况  

Diagnostic value of MYB protein expression in adenoid cystic carcinoma and status of MYB gene copy number

作　　者：霍真[1] 曾瑄 武莎斐 吴焕文 孟云霄 刘媛媛 罗玉凤[1] 曹金伶[1] 梁智勇 

机构地区：[1]中国医学科学院、北京协和医学院、北京协和医院病理科,100730

出　　处：《中华病理学杂志》2015年第8期582-586,共5页Chinese Journal of Pathology

摘　　要：目的探讨MYB蛋白表达在腺样囊性癌诊断及与其他涎腺源性肿瘤鉴别诊断中的意义，并进一步研究MYB基因拷贝数的改变情况。方法收集北京协和医院2000至2014年近15年间活检或手术切除且资料完整的涎腺源性肿瘤89例，其中腺样囊性癌34例，多形性腺瘤10例，基底细胞腺瘤10例，上皮肌上皮癌10例，基底细胞腺癌9例，黏液表皮样癌8例，癌在多形性腺瘤中4例，多形性低度恶性腺癌4例。采用免疫组织化学EnVision法检测34例腺样囊性癌和55例非腺样囊性癌中MYB蛋白的表达情况，并对MYB阳性表达的病例通过荧光原位杂交（FISH）方法检测MYB基因拷贝数。结果MYB在34例腺样囊性癌和55例非腺样囊性癌中阳性表达率分别为82．4％（28／34）和9．1％（5／55），差异有统计学意义（P〈0．01）。2例（2／18）腺样囊性癌发现MYB基因拷贝数增加，非腺样囊性癌组病例未发现该基因拷贝数改变，差异无统计学意义（P=0．435）。结论MYB蛋白表达对腺样囊性癌的诊断及鉴别诊断具有一定价值，可通过MYB免疫组织化学法帮助确诊腺样囊性癌。此外，在少数腺样囊性癌病例中发现有MYB基因扩增，不是导致MYB蛋白过表达的主要因素。Objective To explore the diagnostic value of MYB protein expression for adenoid cystic carcinoma and its differential diagnosis from other salivary gland tumors, and to further investigate the status of MYB gene copy number. Methods MYB expression was studied by immunohistochemistry in 34 adenoid cystic carcinomas, 55 non-adenoid cystic carcinomas （other salivary gland tumors） including 10 pleomorphic adenomas, 10 basal cell adenomas, 10 epithelial-myoepithelial carcinomas, 9 basal cell adenocarcinomas, 8 mucoepidermoid carcinomas, 4 carcinoma in pleomorphic adenomas, and 4 polymorphous low-grade adenocarcinoma. MYB gene copy number status was detected by FISH in MYB protein-positive cases. Results 82.4% （28/34） of adenoid cystic carcinomas were MYB protein-positive, compared with 9. 1% （5/55） of non-adenoid cystic carcinomas, and the difference between the two groups was statistically significant （P〈0.01）. 2/18 of adenoid cystic carcinomas had duplication of MYB gene by FISH, and all non-adenoid cystic carcinomas were negative although the difference was not statistically significant （P = 0. 435 ）. Conclusions MYB protein expression is a useful diagnostic marker for adenoid cystic carcinomas in its separation from other salivary gland tumors. In addition, duplication of MYB gene is no a major mechanism for the MYB protein overexpression.

关 键 词：癌 腺样囊性 涎腺肿瘤 免疫组织化学 原位杂交 荧光 

分 类 号：R739.8[医药卫生—肿瘤]