Twist及YB-1基因表达上调与子宫颈癌上皮间质转化的相关性  

Correlation of Twist and YB-1 up-regulation and epithelial-mesenchymal transition during tumorigenesis and progression of cervical carcinoma

作　　者：李旻 关弘[2] 胡新荣[3] 王鹰 魏茜 杨青凤 

机构地区：[1]广东省深圳市龙华新区人民医院病理科,518109 [2]深圳市第二人民医院病理科 [3]广东医学院肿瘤研究所

出　　处：《中华病理学杂志》2015年第8期594-599,共6页Chinese Journal of Pathology

摘　　要：目的探讨Twist及YB-1基因在子宫颈癌组织中表达的临床病理意义及其与上皮问质转化标志物上皮细胞钙黏蛋白（E-cadherin）表达的相关性。方法选取2008年1月至2013年12月202例子宫颈组织标本，包括正常子宫颈组织50例、子宫颈上皮内瘤变（CIN）100例及宫颈鳞状细胞癌（SCC）52例，采用MaxVision法检测Twist、YB-1蛋白和E-cadherin，并对各组病例的表达差异进行卡方检验、相关性分析及回归模型分析。结果Twist蛋白胞核高表达、YB-1蛋白胞质高表达与子宫颈上皮恶性转化、子宫颈癌的组织级别进展及转移能力提高相关。Twist、YB-1蛋白高表达与E-cadherin低表达相关。回归分析提示Twist蛋白的胞核表达是子宫颈癌进展的独立预测因子。结论Twist和YB-1基因在子宫颈癌中过表达与CIN癌变、子宫颈癌侵袭及转移明显相关，其机制可能是通过（YB-1）-Twist-（E-cadherin）调节关系轴来实现上皮问质转化。Twist和YB-1蛋白过表达可以预测子宫颈癌的恶性转化和转移。Objective To investigate the clinicopathological significance of Twist and YB-1 up- regulation in cervical cancer, and to correlate the expression of the two genes with E-cadherin, a marker of epithelial-mesenchymal transition （EMT）. Methods A total of 202 tissue samples were collected during January 2008 to December 2013, including 50 cases of normal cervical tissues, 100 cases of cervical intraepithelial neoplasia （CIN） and 52 cases of squamous cell carcinoma （SCC）. Twist, YB-1 and E-cadherin expression was investigated by MaxVision. Results Increased expression levels of Twist and YB-1 were found and correlated with the malignant transformation of cervical epithelium, histological progression and metastasis of cervical cancer. In addition, Twist and YB-1 overexpression was also associated with aberrant expression of E-cadherin. Regression analysis revealed that Twist expression was an independent factor for the histological progression of cervical cancer. Conclusions It is suggested that Twist and YB-1 overexpression is significantly linked to cervical cancer tumorigenesis and progression, likely related to EMT through （YB-1）-Twist-（E-eadherin） pathway. Twist and YB-1 may be markers for determining the metastatic potential of cervical cancer.

关 键 词：TWIST转录因子 上皮细胞-间充质细胞转换 宫颈肿瘤 YB-1 上皮细胞钙黏 蛋白 

分 类 号：R737.33[医药卫生—肿瘤]