全肠外营养相关肠黏膜免疫屏障损伤小鼠模型的建立  

Total parenteral nutrition associated intestinal immunity barrier impairment in a mouse model

作　　者：万晓[1] 王新颖[1] 毕景成[1] 李宁[1] 黎介寿[1] 

机构地区：[1]南京大学医学院附属金陵医院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2015年第4期222-225,230,共5页Parenteral & Enteral Nutrition

摘　　要：目的:建立一种稳定有效的小鼠模型,为进一步研究全肠外营养(TPN)相关肠黏膜屏障损伤的机制和干预措施。方法:将22只小鼠随机分为对照组(n=10)和TPN组(n=12)。小鼠经颈内静脉置管后,对照组给予正常饮食,用微量泵持续输注等渗盐水;TPN组给予禁食后用微量泵持续输注PN液。5 d后比较两组小鼠生存率、肠道菌群易位情况、肠道杯状细胞数量以及潘氏细胞变化。结果:两组小鼠的生存率无显著性差异。TPN组肠道菌群淋巴结易位率增加(6/10 vs 1/10,P<0.05),杯状细胞数量减少[(61.6±6.5)个vs(33.0±7.8)个,P<0.01)],隐窝处潘氏细胞内抗菌多肽颗粒明显减少,溶菌酶和黏蛋白2(MUC2)含量显著降低。结论:TPN组小鼠能作为后期研究TPN诱导肠黏膜免疫屏障受损的模型,用于患病机制、药物治疗的观察和研究。Objective： This study was to establish a stable and effective mouse model for further investigation in total parenteral nutrition （TPN） associated intestinal barrier impairment. Methods ： 22 mice were randomly arranged into control group （n = 10 ） or TPN group （n = 12 ）. After the central venous catheter was placed in the fight internal jugular, isotonic saline （ control group ） or parenteral nutrition（ TPN group） was infused through the catheter. Survival rate, intestinal bacteria translocation, the change of goblet cells and Paneth cells were observed. Results： No difference was observed between the two groups in survival rate. TPN group had more bacteria-positive MLN （6/10 vs 1/10, P 〈 0.05） and decreased numbers of goblet cells （61.6 ±6.5 vs 33.0 ±7.8, P 〈 0.01 ）. The antibacterial peptides decreased significantly in TPN group compared with control group. Conclusion： The mouse model is suitable for investigating the pathogenesis and treatment of total parenteral nutrition associated intestinal immunity barrier impairment.

关 键 词：全肠外营养 肠屏障损伤 黏膜免疫 

分 类 号：R574[医药卫生—消化系统] R-332[医药卫生—内科学]