胃癌患者术前腹腔冲洗液中癌胚抗原mRNA的表达水平与手术预后的关系  

The correlation between expression of carcinoembryonic antigen mRNA in preoperative peritoneal lavage fluid and prognosis of operation in patients with gastric cancer

作　　者：缴富斌[1] 袁风仪[1] 杨凡[2] 徐飞[3] 千年松[4] 

机构地区：[1]解放军总参谋部警卫局卫生保健处,北京100017 [2]第四军医大学西京医院外科手术室 [3]解放军第三〇二医院外科 [4]解放军总医院海南分院肿瘤内科,三亚572000

出　　处：《中国医师进修杂志》2015年第8期565-568,共4页Chinese Journal of Postgraduates of Medicine

基　　金：北京市科技新星资助计划（xx2013107）

摘　　要：目的评估胃癌患者术前腹腔冲洗液中癌胚抗原（CEA）mRNA的表达水平与手术预后的关系。方法应用实时荧光定量聚合酶链反应检测68例胃癌患者及30例胃良性病患者术前腹腔冲洗液中CEAmRNA的表达水平。分析其表达水平与胃癌患者临床病理因素以及手术预后之间的关系。结果胃癌患者术前腹腔冲洗液中CEAmRNA表达水平为1．74±0．25，胃良性病患者为0．19±0．04，两者比较差异有统计学意义（P〈0．05）。胃癌患者术前腹腔冲洗液中CEA mRNA的表达水平与浸润深度、分化程度、淋巴结转移、远处转移和TNM分期有关（P〈0．05）。胃癌患者术前腹腔冲洗液CEA mRNA低表达患者（34例），术后1年肿瘤复发转移率显著低于高表达患者（34例）[47．06％（16／34）比82．35％（28／34）]，生存率显著高于高表达患者[79．41％（27／34）比55．88％（19／34）]，差异均有统计学意义（P〈0．05）。结论胃癌患者术前腹腔冲洗液中CEA mRNA与胃癌的临床病理因素密切相关，其高表达提示胃癌患者手术预后差，可能是一个潜在的胃癌预后分子标志物。Objective To investigate the correlation between expression of carcinoembryonic antigen （CEA） mRNA in preoperative peritoneal lavage fluid and prognosis of operation in patients with gastric cancer. Methods The expression levels of CEA mRNA in preoperative peritoneal lavage fluid of 68 patients with gastric cancer and 30 patients with gastric benign tumor were measured by reverse transcription polymerase chain reaction. The correlation between the expression level of CEA mRNA in preoperative peritoneal lavage fluid and clinicopathological features, prognosis of postoperative were analyzed. Results The expression level of CEA mRNA in preoperative peritoneal lavage fluid of patients with gastric cancre was 1.74 ± 0.25 and with gastric benign tumor was 0.19 ± 0.04, and there was statistical difference （P 〈 0.05）. The expression level of CEA mRNA in preoperative peritoneal lavage fluid had a correlation with the tumor infiltration depth, tumor differentiation, lymph node metastasis, distant metastasis and TNM stage （P 〈 0.05）. There was statistical difference in the recurrent / metastasis rate and survival rate 1 year after operation between low （34 cases） and higher （34 cases） expression level of CEA mRNA in preoperative peritoneal lavage fluid of gastric cancer patients： 47.06% （16/34） vs. 82.35% （28/34） and 79.41% （27/34） vs. 55.88% （19/34）, P 〈 0.05. Conclusion The expression of CEA mRNA in preoperative peritoneal lavage fluid has close correlation with the clinicopathologic features, and the high expression of CEA mRNA indicats poor prognosis, indicating that CEA mRNA in preoperative peritoneal lavage fluid could be used as a prognostic marker in patients with gastric cancer.

关 键 词：胃肿瘤 癌胚抗原 预后 

分 类 号：R735.2[医药卫生—肿瘤]