甲强龙对实验性变态反应性脑脊髓炎小鼠的肿瘤坏死因子α和白介素17的影响  

Effect of methylprednisolone on the levels of TNF-α and IL-17 in mice with experimental autoimmune encephalomyelitis

作　　者：李晓峰[1] 李吕力[1] 韦俊杰[1] 李燕华[1] 陈渊[1] 范秉林[1] 张丽香[1] 陈志[1] 肖继东[1] 封浑 韦旋[1] 王夏薇[1] 

机构地区：[1]广西壮族自治区人民医院神经内科,南宁530021

出　　处：《中国临床新医学》2015年第8期714-717,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西医疗卫生重点科研课题(编号:重2010028);广西自然科学基金资助项目(编号:2011GXNSFA018185);国家自然科学基金资助项目(编号:81460192)

摘　　要：目的探讨甲强龙对实验性变态反应性脑脊髓炎(EAE)小鼠的肿瘤坏死因子α(TNF-α)和白介素17(IL-17)的影响。方法将30只雌性C57BL/6J小鼠随机分为甲强龙组、EAE组和正常组,每组10只。EAE模型通过以髓鞘少突胶质细胞糖蛋白多肽35-55(MOG35-55)为抗原诱导。采用蛋白质印迹法(Western Blot)检测小鼠大脑的TNF-α和IL-17表达水平。结果甲强龙组的临床症状较EAE组改善(P<0.05),大脑的TNF-α和IL-17表达水平明显低于EAE组(P<0.05)。结论甲强龙能改善EAE小鼠的症状,其作用机制之一是抑制促炎性因子TNF-α和IL-17的表达。Objective To explore the effects of methylprednisolone on the levels of TNF-αand IL-17 in the central nervous system of mice with experimental autoimmune encephalomyelitis （ EAE ） .Methods 30 female C57BL/6J mice were randomly divided into 3 groups： methylprednisolone-treated group, EAE group and normal group.The EAE model was induced by immunizing mice with myelin oligodendrocyte glycoprotein peptides （ MOG35-55）.The brain tissues were collected and their expressions of TNF-αand IL-17 were detected using Western Blot . Results The neurological deficits of the methylprednisolone-treated mice were lighter than those of the EAE mice （ P〈0.05 ） , while the methylprednisolone-treated mice displayed the lower expressions of TNF-αand IL-17 than the EAE mice did（P〈0.05）.Conclusion Methylprednisolone can protect against EAE in mice by the inhibition of TNF-αand IL-17 .

关 键 词：甲强龙 实验性变态反应性脑脊髓炎 多发性硬化 肿瘤坏死因子Α 白介素17 

分 类 号：R742[医药卫生—神经病学与精神病学]