miR-145对NSCLC细胞增殖和FSCN1蛋白表达的影响研究  

Effect of miR-145 on NSCLC cell proliferation and FSCN1 protein expression

作　　者：牟志民[1] 毛广显 谢远财[1] 彭旭兴[1] 乌达[1] 

机构地区：[1]北京大学深圳医院胸外科,广东深圳518036

出　　处：《中国生化药物杂志》2015年第3期25-27,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：深圳市科技计划项目(JCYJ20140415162338820);深圳市医疗卫生科研项目(201302068)

摘　　要：目的研究miR-145在人非小细胞肺癌细胞A549中的表达及其对A549细胞增殖和靶蛋白FSCN1表达的影响。方法采用分子克隆实验构建pmR-mcherry/miR-145真核表达载体,瞬时转染A549细胞,QPCR法检测miR-145在细胞中的表达水平;Western blot检测miR-145对细胞中FSCN1蛋白表达水平的影响;细胞增殖实验检测miR-145对A549细胞增殖能力的影响。结果成功构建pmR-mcherry/miR-145真核表达载体,转染A549细胞后QPCR检测其可有效表达;Western blot结果显示过表达miR-145后,FSCN1的蛋白水平明显的下调;细胞增殖实验结果显示miR-145能明显抑制肺癌细胞A549的增殖。结论过表达miR-145可抑制A549细胞增殖并下调FSCN1基因的表达水平。Objective To investigate the effect of miR-145 on human lung adenocarcinaoma A549 cell proliferation and FSCN1 expression. Methods pmR-mcherry/miR-145 was constructed and transfected into A549 cell,then the expression of miR-145 and the proliferation of A549 cell were verified by QPCR and MTS assay, respectively.The situation of FSCN1 expression in A549 cell was detected by Western blot.Results pmR-mcherry/miR-145 vector was constructed successfully,and QPCR results indicated that miR-145 expressed effectively.Western blot results showed that FSCN1 was one of the targets of miR-145 in A549 cell.MTS assay results indicated that miR-145 inhibited the proliferation of A549 cell.Conclusion Overexpression of miR-145 can inhibit the proliferation of A549 cell, and FSCN1 was one of its target.

关 键 词：MIR-145 FSCN1 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]