黄芩苷对牙周炎大鼠牙移动保持阶段VEGF、BMP-2及IL-1β表达的影响  

Effect of baicalin on expression of VEGF,BMP-2 and IL-1β in rat mobile dental periodontitis maintained phase

作　　者：李玲[1] 

机构地区：[1]莱芜市人民医院口腔科,山东莱芜271199

出　　处：《中国生化药物杂志》2015年第3期32-35,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的观察黄芩苷对牙周炎大鼠牙移动保持阶段血管内皮生长因子(vascular endothelial growth factor,VEGF)、骨形态发生蛋白2(bone morphogenetic protein,BMP-2)和白介素1β(interleukin,IL-1β)表达的影响,探讨黄芩苷对牙周组织改建的作用机制。方法 80只SD大鼠,随机分为4组,每组20只。空白组不作处理,模型组建立牙周炎模型,对照组与观察组均建立牙周炎模型,继而建立牙移动模型,观察组给予黄芩苷,空白组和对照组给予等量生理盐水,分别于1、3、6、12、18d时处死大鼠进行免疫组化染色,观察牙周组织VEGF、BMP-2和IL-1β的表达变化。结果空白组大鼠BMP-2阳性表达不高,模型组BMP-2表达增强,对照组和观察组牙周组织BMP-2的表达在加力结束后逐渐降低,但相同时间点比较,观察组BMP-2表达量低于对照组(P<0.05)。对照组和观察组牙周组织VEGF表达量在加力结束后均较模型组显著降低,相同时间点比较,观察组VEGF表达量均高于对照组(P<0.05)。空白组IL-1β阳性表达不明显,模型组IL-1β阳性表达明显增多,对照组和观察组牙周组织IL-1β表达量在加力结束后逐渐降低,相同时间段组比较,观察组IL-1β表达量明显低于对照组(P<0.05)。结论黄芩苷可显著减少牙周炎大鼠牙移动后牙周组织中IL-1β表达,降低炎症反应,抑制骨吸收;增加牙周组织中BMP-2和VEGF表达,促进牙周组织血管形成和成熟,这可能是黄芩苷促进牙周炎大鼠正畸牙移动后保持阶段内牙周组织改建的作用机制之一。Objective To observe effect of baicalin on VEGF, BMP-2 and IL-1βexpression in rat mobile dental periodontitis maintained phase, and explore the mechanism of baicalin on periodontal tissue remodeling.Methods 80 SD rats were randomly divided into four groups,20 rats in each group.The blank group weren’t giren any treatment, model group were built with periodontitis, control group and observation group were built with periodontitis and then tooth movement model.The observation group were given baicalin, blank group and control group were given the same volume of saline.The rats were killed at 1d, 3d, 6d, 12d, 18d, then samples of the maxilla were taken to make tissue sections and immunohistochemical staining. VEGF, BMP-2 and IL-1βexpression were observed in periodontal tissue.Results The BMP-2 expression isn’t obvious in blank group, but was obvious in model group, and gradually reduced at the end of the afterburner; BMP-2 expression in experimental group were higher than that in control group at the same point （ P〈0.05 ） .The VEGF expression in control group and experimental group gradually reduced at the end of the afterburner; VEGF expression in experimental group were lower than that in control group at the same point （ P〈0.05 ） .The IL-1βexpression wasn’t obvious in blank group, but was obvious in model group.The IL-1βexpression in control group and experimental group gradually reduced at the end of the afterburner;IL-1βexpression in experimental group were lower than that in control group at the same point （ P〈0.05 ） .Conclusion Baicalin can significantly increase the expression of IL-1βin periodontal tissues of periodontitis rats after moving teeth, reduce inflammation, inhibit bone resorption, and also increase BMP-2 and VEGF expression, promote periodontal vessel formation and maturation, which may be one of the mechanisms of baicalin to promote periodontal tissue remodeling in periodontitis rats at holding phase after orthodontic tooth movement.

关 键 词：牙周炎 黄芩苷 VEGF BMP-2 IL-1Β 

分 类 号：R781.42[医药卫生—口腔医学]