L-岩藻糖的生产菌种选育及发酵工艺优化  

Strain Screening and Fermentation Production of L-Fucose by Enterobacter sp.

作　　者：黄宜兰[1] 王岩[1] 陈少欣[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2015年第8期823-826,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2012ZX09301002-003)

摘　　要：以肠杆菌属(Enterobacter sp.)SIPI-10为出发菌株,经紫外与亚硝基胍(NTG)诱变,筛得1株L-岩藻糖高产突变株SIPI-N79,产量为1 276 mg/L。通过优化发酵培养基组成,得到优化配方(g/L):甘油30.0,胰蛋白胨LP0042 5.0,CaCO3 5.0,L-岩藻糖的摇瓶发酵产量为1 582 mg/L。进一步在5 L发酵罐中进行分批补料培养,当补加150 g葡萄糖时,L-岩藻糖的产量达到4 553 mg/L,较摇瓶发酵水平提高显著。The starting strain Enterobacter sp. SIPI-10 was mutated with UV and NTG for the production of L-fucose. A high-yield mutant strain SIPI-N79 was obtained and the yield of L-fucose was 1 276 mg/L. By optimizing fermentation medium, SIPI-N79 produced 1 582 mg/L L-fucose in shaking flask contained （g/L） glycerol 30,0, peptone LP0042 5.0, CaCO3 5.0. Furthermore, a fed-batch culture in a 5 L fermentor was conducted. The yield of L-fucose reached to 4 553 mg/L with feeding 150 g of glucose, which showed a significant increase in yield compared with that in shaking flask culture.

关 键 词：L-岩藻糖 肠杆菌属 菌种选育 发酵 

分 类 号：TQ46[化学工程—制药化工]