Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina  

花生四烯酸生产菌株高山被孢霉油脂特性分析（英文）

作　　者：邬文嘉 颜佳铖 纪晓俊 张鑫 商静生 孙丽娜 任路静 黄和 

机构地区：[1]State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University [2]Faculty of Science, Hong Kong Baptist University [3]College of Sciences, Nanjing Tech University

出　　处：《Chinese Journal of Chemical Engineering》2015年第7期1183-1187,共5页中国化学工程学报（英文版）

基　　金：Supported by the National Science Foundation for Distinguished Young Scholars of China(21225626);the National Basic Research Program of China(2011CBA00800);the National Natural Science Foundation of China(21376002,21476111);the Natural Science Foundation of Jiangsu Province(BK20131405);the National High-Tech R&D Program of China(2014AA021703);the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：Mortierella alpina has been considered as the most effective producer of arachidonic acid(ARA)-rich oil. It was found that several methods could improve the percentage of ARA in total lipids successfully, as they activated the desaturation system on the endoplasmic reticulum. Additionally, in M. alpina the ARA exists in several forms, such as triacylglycerol(TAG), and diacylglycerol(DAG). These forms are caused by different acyltransferases and they determine the nutrient value of the microbial oil. However, few works revealed detailed fatty acid distribution among lipid classes, which to some extent impeded the accurate regulation in ARA accumulation. Herein, this paper gives information on the accumulation process of main lipid classes and the changes of fatty acid composition in these lipids during ARA accumulation period in M. alpina. The result demonstrates that TAG was the dominant component of the total lipids, and it is the main form for ARA storage. The ARA enrichment stage occurred during 168–192 h when the amount of total lipids maintained steady. Further analysis indicated that the newly formed ARA-TAG might come from the incorporation and modi fication of saturated and monounsaturated fatty acids in other lipid classes. This work could be helpful for further optimization of ARA-rich TAG production.Mortierella alpina has been considered as the most effective producer of arachidonic acid （ARA）-rich oil. It was found that several methods could improve the percentage of ARA in total lipids successfully, as they activated the desaturation system on the endoplasmic reticulum. Additionally, in M. alpina the ARA exists in several forms, such as triacylglycerol （TAG）, and diacylglycerol （DAG）. These forms are caused by different acyltransferases and they determine the nutrient value of the microbial oil. However, few works revealed de- tailed fatty acid distribution among lipid classes, which to some extent impeded the accurate regulation in ARA accumulation, Herein, this paper gives information on the accumulation process of main lipid classes and the changes of fatty acid composition in these lipids during ARA accumulation period in M. alpina. The result demonstrates that TAG was the dominant component of the total lipids, and it is the main form for ARA storage. The ARA enrichment stage occurred during 168-192 h when the amount of total lipids maintained steady. Further analysis indicated that the newly formed ARA-TAG might come from the incorporation and modification of saturated and monounsaturated fatty acids in other lipid classes. This work could be helpful for further optimization of ARA-rich TAG production.

关 键 词：Arachidonic acidLipid characterizationTriacylgiycerol Mortierella alpina 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]