人MCL1不同亚型在结肠癌中表达变化的临床意义  

Clinical significance of human MCL1 expression in colon cancer tissues

作　　者：施莉[1] 韦炜[1] 向华[1] 薛向阳[2] 叶璐璐[2] 范波[3] 

机构地区：[1]浙江省金华市人民医院消化科,321000 [2]浙江省温州医科大学基础医学院,325035 [3]浙江省金华市人民医院检验科,321000

出　　处：《中华全科医学》2015年第10期1576-1578,共3页Chinese Journal of General Practice

基　　金：浙江省科技厅课题(2014C33140)

摘　　要：目的以MCL1基因为例,研究不同亚型在结肠癌组织中差异表达的临床意义。方法采用RT-PCR和WB分别检测正常结肠组织、结肠腺瘤、结肠腺癌组织以及对应的癌旁组织各20例中MCL1基因3种mRNA剪接异构体和3种蛋白亚型的表达水平,进而通过χ2检验分析了这些亚型表达差异在213例肿瘤临床病例中与肿瘤病理特征之间的相关性。结果 MCL1基因亚型1在结肠肿瘤组织及对应旁组织中的表达均高于正常组(mRNA,P=0.009;蛋白,P=0.022),且腺癌组中的表达亦高于腺瘤组(mRNA,P=0.004;蛋白,P=0.034)。亚型2在结肠肿瘤组织及对应旁组织中的表达均高于正常组(mRNA,P=0.002;蛋白,P=0.011),且腺癌组中的表达亦高于腺瘤组(mRNA,P<0.001;蛋白,P=0.007)。亚型3在结肠肿瘤组织及对应旁组织中的表达均高于正常组(mRNA,P=0.027;蛋白,P=0.045),但肿瘤组与对应旁组织之间以及腺癌组与腺瘤组之间的比较均无统计学意义。此外MCL1亚型1和亚型2对结肠癌肿瘤恶性程度呈正相关,而亚型3可以作为肿瘤早期诊断的标志物,但其表达水平与恶性程度无相关性。结论在临床评估结肠癌恶性程度过程中,位于核表达的MCL1亚型2可以作为有效的诊断指标,本研究为MCL1引物设计以及抗体选择提供一定参考价值。Objective To investigate the clinical significance of differential human MCL1 isoform expressed in colorectal cancer tumor tissues. Methods RT-PCR and Western blot were performed to detect three mRNA splicing variants and protein isoforms of MCL1 gene in normal colon tissues, colorectal adenoma, colorectal adenocarcinoma and cancer-adjacent tissue（20 cases for each group）. Then the expression of these variants in 213 cases of clinical samples was detected and their relationship with thepathological characterization of the colon tumors was analyzed by using Chi-square test. Results The isoform 1 expressed higher in the colon tumors and cancer-adjacent tissues than in normal control（mRNA, P = 0. 009 ; protein, P = 0. 022 ）, and expressed higher in the adenocarcinoma group than adenoma group （ mRNA, P = 0.004 ;protein,P = 0.034）. The isoform 2 expressed higher in the colon tumors and cancer-adjacent tissues than in normal control（mRNA, P = 0. 002; protein, P = 0.011 ）, and expressed higher in the adenocarcinoma group than adenoma group（ mRNA,P 〈 0.001 ;protein, P = 0. 007 ）. The isoform 3 expressed higher in the colon tumors and cancer-adjacent tissues than in normal control（ mRNA, P = 0. 027 ; protein, P = O. 045 ）, but no statistical significance of the comparison between tumor group andcancer-adjacent groups as well as the comparison between adenocarcinoma group and adenoma group. Meanwhile, the presence of the isoforms 1 and 2 but not 3 of MCL1 expression were demonstrated as a positive correlation with the malignancy level of colon tumor while the isoform 3 could use to be a early biomarker for colon tumor diagnosis. Conclusion The isoform 2 expressed in nucleus can be used as an efficient biomarker for the evaluation of the malignancy level of colon tumor in clinical diagnosis. Our study provide an evidence for MCL1 primers design and antibod- ies selection in coming related research.

关 键 词：结肠癌 MCL1 亚型 RNA剪接异构体 蛋白亚型 

分 类 号：R735.35[医药卫生—肿瘤]