鹿衔草多糖不同提取方法的比较  

Compare the Extraction Methods of Pyrola Polysaccharide

作　　者：董晶晶[1] 张杰[2] 刘炯[2] 杨云[2] 

机构地区：[1]郑州市第六人民医院,河南郑州450000 [2]河南中医学院,河南郑州450046

出　　处：《中医学报》2015年第8期1173-1175,共3页Acta Chinese Medicine

摘　　要：目的:比较不同提取方法对鹿衔草多糖提取效率的影响。方法:以多糖得率和多糖含量为指标,采用正交试验的方法对水提法、碱提法、酶提法进行比较。结果:水提法的最佳工艺为药材粉末加入30倍量水,100℃回流提取2次,每次3 h,多糖得率为14.56%,多糖含量为19.97%;碱提法的最佳工艺为药渣加入10倍量的浓度为0.08 mol·L-1Na OH溶液,4℃浸提2h,提取4次,多糖得率为16.75%,多糖含量为16.15%;酶提法的最佳工艺为加体积分数4%果胶酶、调整p H到4.0,酶解温度40℃,多糖得率为16.57%,多糖含量为21.97%。结论:酶提法为最佳提取方法。Objective：Compare the different extract methods of Pyrgla Polysaccharide, Methods：Set polysaccharide yield and content as indices, compare the method of water extraction, alkali extraction and enzyme extraction by means of orthogonal test. Results ： The best crafts of water extraction is add 30 times water to medicinal powder,reflux twice in 100 ℃ ,3 h every time,polysaccharide yield is 14. 56%, polysaccharide content is 19.97% ;The best crafts of alkali extraction is add 10 times 0.08 mol · L- l NaOH solution to dregs, ex- tract 4 times in 4 ℃ ,2 h every time,polysaecharide yield is 16.75% ,polysaecharide content is 16.15% ;The best crafts of enzyme ex- traction is add 4% pectinase, adjust Ph to 4.,0 and temperature to 40 ℃ , polysaccharide yield is 16.57% , polysaccharide content is 21. 97%. Conclusion：Enzyme extraction is the best extract method.

关 键 词：鹿衔草多糖 水提法 碱提法 酶提法 正交试验 

分 类 号：R284.2[医药卫生—中药学]