红松4个组织的转录组数据分析与次生代谢产物的表达差异初探  

Differential Gene Expression Analysis on Secondary Metabolites and Transcriptome Sequencing to Four Tissues of Pinus koraiensis

作　　者：张振[1] 张含国[1] 周宇[1] 张磊[1] 于宏影[1] 张莉[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《林业科学研究》2015年第4期597-603,共7页Forest Research

基　　金：林业公益性行业科研专项"红松果材兼用林良种选育与定向培育技术研究"(201204320)

摘　　要：红松（PinusKoraiensis）为松科松属乔木，主要分布在中国东北东部，俄罗斯远东南部及朝鲜半岛，日本岛也有零星分布。红松是中国Ⅱ级重点保护野生植物，既是优良的用材树种，又是珍贵的食用干果和油料树种。红松树干圆满通直，叶5针一束，球果两年成熟，花期5-6月，种子在次年9—10月成熟。红松木材可作建筑、造纸，树皮中含有松树脂.This research compared the transcriptomes of four different tissues of Pinus koraiensis by using RNA-seq technique and a lot of differentially expressed unigenes were obtained. A total of 21.3 GB of data was obtained and 41 476 expressed unigenes were received after assembling. Through GO and COG classification, the commented and transcribed sequences were classified into 24 or 56 functional categories, which referred to many biochemical proces- ses such as material and energy metabolism, signal transduction, transcriptional regulation and defense reaction. Through pathway enrichment analysis, the pathways associated with grease synthetic fatty acid biosynthetic and ter- penoids synthesis were found. Two unigenes of metabolic pathways in KEGG database were compared and 16 key enzyme genes of fatty acid synthesis in other species homologous and 34 key enzyme genes of terpenoid biosynthetic pathway were obtained. By high-throughput sequencing and bioinformatics research, a large number of Pinus ko- raiensis transeriptome unigenes was obtained. This research provides references for the research on the genes related to secondary metabolites synthesis in Pinus koraiensis,

关 键 词：红松 转录组 基因注释 次生代谢途径 

分 类 号：S791.245[农业科学—林木遗传育种]