非小细胞肺癌BALF中RAR-β基因甲基化与p53突变检测及相关性研究  

Correlation between RAR-β gene methylation and p53 gene mutation in bronchoalveolar lavage fluid in non-small-cell lung cancer

作　　者：李琪[1] 肖贵华 程长浩 常芬[1] 

机构地区：[1]武汉科技大学附属普仁医院呼吸内科,武汉430081

出　　处：《重庆医学》2015年第23期3206-3209,共4页Chongqing medicine

基　　金：湖北省教育厅基金项目(Q20131106)

摘　　要：目的探讨非小细胞肺癌患者支气管肺泡灌洗液(BALF)中RAR-β基因甲基化与p53基因突变检测的临床意义及二者的相关性。方法收集非小细胞肺癌患者(肺癌组)85例及良性疾病患者(良性疾病组)70例的BALF标本,采用甲基化特异性PCR(MSP)方法检测BALF中的RAR-β基因甲基化,PCR结合DNA测序法检测p53基因突变。结果肺癌组BALF中RAR-β基因甲基化率及p53基因突变率分别为49.4%、36.5%,均显著高于良性疾病组(P<0.01);(Ⅰ+Ⅱ)期RAR-β基因甲基化率(32.5%)高于同期p53基因突变率(12.5%)(P<0.05);(Ⅲ+Ⅳ)期RAR-β基因甲基化率及p53基因突变率均显著高于(Ⅰ+Ⅱ)期(P<0.01);发生RAR-β基因甲基化的肺癌患者p53基因突变率更高(P<0.01);发生p53基因突变的肺癌患者RAR-β基因甲基化率更高(P<0.01)。结论检测非小细胞肺癌患者BALF中RAR-β基因甲基化与p53基因突变有助于肺癌的诊断。Objective To investigate the clinical significance and correlation between RAR-βgene methylation and p53 gene mutation in bronchoalveolar lavage fluid（BALF）in non-small-cell lung cancer.Methods BALF samples from 85 lung cancer patients（lung cancer group）and 70cases（benign lung diseases group）with benign lung diseases were collected.RAR-βgene methylation in BALF samples was detected by methylation-specific PCR（MSP）,and p53 gene mutation was detected by PCR and DNA sequencing method.Results The rate of RAR-βmethylation and p53 mutation in BALF in lung cancer were 49.4% and 36.5%,respectively.Both were higher than in benign lung diseases group（P〈0.01）.RAR-βmethylation rate（32.5%）of patients with TNM stages（Ⅰ＋Ⅱ）（32.5%）was higher than the p53 mutation rate（12.5%）over the same stages（P〈0.05）.RAR-βmethylation rate and p53 mutation rate of patients with stages（Ⅲ＋Ⅳ）were higher than those with stages（Ⅰ＋Ⅱ）（P〈0.01）.p53 mutation rate in lung cancer patients with RAR-βmethylation was higher than those with unmethylated（P〈0.01）.RAR-βmethylation rate of lung cancer patients with p53 mutation was higher than those without p53mutation（P〈0.01）.Conclusion Detection of RAR-βmethylation and p53 mutation in BALF contribute to the diagnosis of lung cancer.

关 键 词：癌 非小细胞肺 支气管肺泡灌洗液 甲基化 RAR-β基因 p53基因 

分 类 号：R734.2[医药卫生—肿瘤]