胰岛素样生长因子-Ⅰ在豚鼠形觉剥夺性近视眼RPE-脉络膜的表达  

Effects on expression of IGF- Ⅰ mRNA by form deprivation myopia in retinal pigment epithelium- choroid in guinea pig

作　　者：田军[1] 吕勇[1] 

机构地区：[1]郑州市第二人民医院眼科,郑州450000

出　　处：《医药论坛杂志》2015年第7期41-43,共3页Journal of Medical Forum

摘　　要：目的建立豚鼠形觉剥夺性近视眼模型,研究IGF-ⅠmRNA在RPE-脉络膜的变化规律,以探讨IGF-Ⅰ与形觉剥夺性近视发生的关系。方法实验用三色豚鼠60只,断乳后1周,半透明眼罩遮盖左眼,右眼为对照眼。遮盖4周实验结束时,RT-PCR检测后极部RPE-脉络膜中IGF-ⅠmRNA的表达水平。结果形觉剥夺4周时遮盖眼形成明显的近视,遮盖眼后极部RPE-脉络膜IGF-ⅠmRNA表达量为(1.871±0.173),对照眼表达量为(1.428±0.155),遮盖眼较对照眼表达量升高,两者相比差异具有显著性意义(t=-5.219,P=0.004)。结论 IGF-Ⅰ参与豚鼠形觉剥夺性近视形成过程。Objective To investigate the changes of IGF - I expression levels in posterior retinal pigment epithelium - choroid of form deprivation myopia in guinea pig. Methods FDM was established in 60 1 - week - old guinea pigs, ret- ino - scopic refraction was performed. The expression of IGF - I mRNA was detected via single step reverse transcription - polymerase chain reaction. Results Form - deprivation eyes were significantly more myopia than control eyes. The levels of IGF - I mRNA expression in occluded eyes was higher in posterior retinal pigment epithelium - choroid than that of the control eyes （ P 〈 0. 05 ）. Conclusion The results suggested that IGF - I participated in the development of form deprived myopia.

关 键 词：形觉剥夺性近视 RPE-脉络膜 胰岛素样生长因子-Ⅰ 

分 类 号：R-332[医药卫生]