miR-200c靶向DNMT1基因对人胶质瘤细胞增殖的影响  

miR-200c Inhibits the Proliferation of Glioma Cells Through Targeting DNMT1

作　　者：刘爱群[1] 余青云[1] 彭忠兴[1] 黄叶青[1] 刁胜朋[1] 程静[1] 王文涛[1] 洪铭范[1] 

机构地区：[1]广东药学院附属第一医院神经内科,广东广州510008

出　　处：《医学临床研究》2015年第7期1361-1363,共3页Journal of Clinical Research

摘　　要：【目的】探讨miR-200c靶向调控DNA甲基转移酶1（DNMT1）对人胶质瘤U251细胞增殖的影响。【方法】采用实时荧光定量PCR（quantitative real—time PCR，qRT-PCR）检测24例胶质瘤组织和大脑正常组织中miR-200c与DNMT1的表达改变；将miR-200cmimics转染于胶质瘤U251细胞，以DNMT1 siRNA为阳性对照，采用qRT—PCR检测外源过表达miR-200c对DNMT1 mRNA表达水平的影响；采用MTT和法检测外源高表达miR-200c时胶质瘤U251细胞增殖能力的影响。【结果】qRT-PCR检测结果显示，miR-200c在24例胶质瘤组织中的表达水平较大脑正常组织明显下调，DNMT1在胶质瘤组织中的表达水平较大脑正常组织明显上调；外源过表达miR-200c下调胶质瘤U251细胞DNMT1mRNA的表达水平（P〈0．05），抑制胶质瘤U251细胞的生长（P〈0．05）。【结论】miR-200c通过靶向调控DNMT1抑制人胶质瘤细胞的增殖。[Objective] To explore whether miR-200c inhibits the proliferation of glioma cells through targeting DNMT1. [Methods] Quantitative real-time polymerase chain reaction （qRT-PCR） was employed for detecting the expressions of miR-200c and DNMT1 in glioma and normal brain specimens respectively, U251 cell was transfected with miR-200c mimics and DNMT1 si-RNA as a positive control. Then qRT-PCR was conducted for detecting the cellular expressions of DNMT1 mRNA regulated by miR-200c. And cell proliferation was assayed by methyl thiazolyl tetrazolium （MTT）. [Results] miR-200c was down-regulated in glioma tissues while DNMT1 up-regulated in glioma tissues. The cellular level of DNMT1 mRNA was inhibited by restoring miR-200c or knocking down DNMT1. And an over-expression of miR-200c or a knock-down DNMT1 could inhibit the cell proliferation. [Conclusion] miR-200c inhibits cell proliferation through targeting DNMT1 in glioma ceils.

关 键 词：微RNAS DNA(胞嘧啶-5-)-甲基转移酶/遗传学 神经胶质瘤/遗传学 神经胶质瘤/病理学 

分 类 号：R730.264[医药卫生—肿瘤]