显性矮秆基因Rht10对小麦籽粒灌浆特性的影响  

Effects of Rht10,a Dominant Dwarfing Gene on Grain Filling of Wheat

作　　者：李骞骞 张卫东[1] 高建华 张淑英 姜晓丽 高庆荣[1] 

机构地区：[1]山东农业大学农学院/作物生物学国家重点实验室,山东泰安271018 [2]沂南县烟草专卖局(分公司),山东沂南276000 [3]德州市农业局种子管理处,山东德州253016

出　　处：《山东农业科学》2015年第7期33-37,共5页Shandong Agricultural Sciences

基　　金：国家"863"计划项目--强优势杂交种的创制与应用(2011AA10A106);小麦新品种培育与扩繁项目(2011BAD35B03)

摘　　要：为了探究显性矮秆基因Rht10降低粒重的原因,本研究以Rht10鲁麦15、鲁麦15近等基因系为材料,分析其籽粒形成期粒重的变化,并利用Logistic方程拟合灌浆过程。结果显示,在籽粒形成过程中,二者粒重的变化均呈＂S＂型曲线,但Rht10鲁麦15粒重均低于鲁麦15,降低幅度在1.17%-35.71%之间;灌浆速率的变化均呈单峰曲线,灌浆中期Rht10鲁麦15的灌浆速率明显高于鲁麦15。Logistic方程拟合结果表明,与鲁麦15相比,Rht10鲁麦15的最大灌浆速率出现时间提早1.57 d,最大灌浆速率高0.26 g/（百粒·d）,籽粒灌浆期短10.08 d,灌浆活跃持续天数短11.12 d,灌浆中期、后期的时间明显低于鲁麦15。由此可见,Rht10基因对粒重的影响贯穿于籽粒发育的整个过程。该基因提高了灌浆期的最大灌浆速率,但缩短了灌浆时间,尤其是缩短了灌浆中期、后期的时间,造成粒重降低。In order to study the mechanisms of reducing grain weight of RhtlO, a dormant dwarfing gene in Triticum aestivum, two near isogenic lines, Lumai 15 and Rht10 Lumai 15 were selected as experimental materials and the dynamic changes of grain weight during grain filling stage were measured and analyzed by Logistic equation fitting. The results showed that the grain weight of the two lines showed ＂S＂ shape curve; the grain weight of Rht10 Lumai 15 was always lower than that of LumailS, and the decreasing amplitudes were between 1.17% and 35.70%. The changes of grain filling rate showed a single peak curve, and the grain filling rate in Rht10 Lumai 15 was higher than that in Lumai 15 during the middle stage. The results of Logistic equation fitting showed that compared with Lumai 15, the appearance of maximum grain filling rate in Rhtl0 Lumai 15 was advanced with 1.57 days;the maximum grain filling rate was 0.26 g per 100 grains one day higher;the grain filling duration was 10.08 days shorter, and the active growing duration was 11.12 days shorter. The duration of middle and later stage of grain filling in RhtlO Lumai Lumai 15. Thus, the effects of RhtlO on grain weight were involved in the The gene increased the maximum grain filling rate and grain filling period, 15 was obvious shorter than that in whole grain development duration. especially the middle and late periwhich might result in the reduction of wheat grain weight.

关 键 词：Rht10近等基因系 鲁麦15 Logistic曲线 灌浆速率 灌浆时间 

分 类 号：S512.101[农业科学—作物学]