微泡增强的超声空化阻断肿瘤微循环的病理观察  

Pathological changes of tumor microcirculation obstruction by microbubbles mediated ultrasound cavitation

作　　者：钟渝[1] 刘政[2] 

机构地区：[1]中国人民解放军第324医院肾内科,重庆400020 [2]第三军医大学新桥医院超声科,重庆400037

出　　处：《中国医学影像技术》2015年第8期1131-1134,共4页Chinese Journal of Medical Imaging Technology

基　　金：重庆市基础与前沿计划项目(cstc2013jcyjA0913);成都军区医学科学技术研究计划项目面上项目(C14022)

摘　　要：目的探讨微泡增强的超声空化阻断肿瘤微循环的病理机制。方法建立兔肝VX2转移瘤模型18只,随机分为超声微泡组(n=6)、单纯超声组(n=6)及假照组(n=6)。对超声微泡组经耳缘静脉注射微泡,并进行超声辐照;对单纯超声组以生理盐水代替微泡,并进行超声辐照;对假照组注射生理盐水,并进行超声假照。观察辐照后各组病理变化。结果大体解剖观察,超声微泡组辐照面可见较多出血点;单纯超声组及假照组辐照后肿瘤组织均未见明显改变。光镜下观察,超声微泡组肿瘤组织可见大片出血,血管内皮细胞损伤、连续性中断,红细胞外溢;单纯超声组可见局灶性小片状出血,血管内皮尚完整;假照组肿瘤组织未见明显出血,血管内皮完整。电镜下观察,超声微泡组肿瘤血管内皮破损严重,可见大量红细胞渗出,线粒体肿胀及吞饮小泡;单纯超声组及假照组仅见内质网略水肿。结论微泡增强的超声空化阻断兔VX2肝脏肿瘤微循环的机制可能为血管机械性损伤。Objective To explore the pathological mechanism of microbubbles mediated ultrasound cavitation obstructed tumor microcireulation. Methods Totally 18 New Zealand rabbits bearing liver VX2 tumor were randomly divided into 3 groups （each n= 6）. The microbubbles injection and ultrasound irradiation were performed in mirobubbles group （US-k-MB group）. The normal saline injection and ultrasound irradiation were performed in simple ultrasound group （US group）. And the normal saline injection and sham ultrasonic irradiation were performed in sham-irradiated group. The pathological changes were observed. Results The gross anatomy showed significant hemorrhage of target region in US-）-MB group, while no significant changes in US and sham-irradiated groups. Pathological observation with light microscope showed sig- nificant hemorrhage, erythrocyte spillover, vascular endothelial interrupt and cells injured in USd-MB group; few hemor- rhage and mild injury of endothelium in US group; unbroken endothelium and insignificant hemorrhage in sham-irradiated group. Pathological observation with electronic microscope showed badly broken endothelium, significant hemorrhage; swelling of mitoehondrion and pinocytosis vesicles in US ＋ MB group, only edema of endoplasmic reticulum in US and sham-irradiated groups. Conclusion The pathological mechanism of microbubbles mediated ultrasound cavitation on tumor microcirculation obstruction maybe vascular mechanical damage.

关 键 词：微泡 空化 超声学 新生血管化 病理性 动物实验 

分 类 号：R-332[医药卫生] R445.1