雌激素孕激素联合紫杉醇对人卵巢癌细胞生长调控及Drosha表达的影响  

Effect of estrogen or progesterone combined with paclitaxel on human ovarian cancer cell growth and Drosha expression

作　　者：杨云洁[1] 韩克 谢玉莲 

机构地区：[1]江苏省淮安市妇幼保健院盆底中心,223002 [2]南京市鼓楼医院妇科

出　　处：《中华肿瘤杂志》2015年第8期578-584,共7页Chinese Journal of Oncology

基　　金：南京市医学重点科技发展项目（ZKX10010）

摘　　要：目的探讨雌激素、孕激素和紫杉醇对人卵巢癌细胞生长调控及Drosha表达的影响。方法雌激素、孕激素和紫杉醇体外作用人卵巢癌细胞，四甲基偶氮唑蓝（MTT）法检测细胞抑制率，流式细胞仪检测细胞凋亡率和细胞周期，实时荧光定量PCR和Westernblot法检测DroshamRNA和蛋白的表达。结果对照组、雌激素组、孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的细胞生长抑制率分别为0％、（31．53±8．21）％、（25．22±15．50）％、（46．71±4．25）％、（69．46±3．71）％和（47．35±39．02）％，雌激素组、孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的细胞生长抑制率与对照组比较，差异均有统计学意义（均P〈0．05）。相对于ER（-）的卵巢癌细胞，雌激素组、孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的DroshamRNA表达水平分别为1．62±0．10、1．60±0．10、1．75±0．16、1．95±0．20和1．53±0．06，与对照组（1．00）差异均有统计学意义（均P〈0．05）；相对于ER（＋）的卵巢癌细胞，雌激素组、孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的DroshamRNA表达水平分别为1．03±0．14、1．60±0．09、1．75±0．16、1．60±0．10和1．53±0．06，孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的DroshamRNA表达水平与对照组差异均有统计学意义（均P〈0．05）。相对于ER（-）的卵巢癌细胞，对照组、雌激素组、孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的Drosha蛋白表达水平分别为0．25±0．05、0．87±0．30、0．85±0．38、1．30±0．21、1．75±0．83和1．62±0．82，雌激素组、孕激素组、紫杉醇组、雌激素联合紫杉醇组和孕激素联合紫杉醇组的Drosha蛋白表达水平与�Objective To investigate the effect of estrogen （E2） , progesterone （ P4）, and paclitaxel （taxol） on the growth of primary human ovarian cancer ceils in vitro and the expression of Drosha. Methods Human ovarian cancer cells were treated with estrogen, progesterone or in combination with paclitaxel in vitro. The inhibition rate of ovarian cancer cells was assessed by methyl thiazolyl tetrazolium （MTF） assay. Apoptosis rate and cell cycle were determined by FACS analysis.The relative abundence of Drosha expression was detected by real-time quantitative PCR （qRT-PCR） and Western blotting. Results The inhibition rateof the estrogen group, progesterone group, paclitaxel group, E2 （＋）Taxol group, P4 （ ± ） Taxol group was （31.53±8.21）%, （25.22±15.50）%, （46.71±4.25）%, （69.46±3.71）%, and （47.35±39.02）%, respectively, significantly higher than that of the control group （0%, P〈0.05 for all）. Relative to the ER （-） in ovarian cancer cells, Drosha mRNA expression level of estrogen group, progesterone group, paclitaxel group, E2（＋）Taxol group,and P4（ ±）Taxol group was 1.62±0.10,1.60±0.10,1.75±0.16,1.95±0.20, and 1.53±0.06, respectively, significantly higher than that of the control group （ 1.00,P〈0.05 for all）. Relative to the ER （ ± ） in ovarian cancer cells, the Drosha mRNA expression level of estrogen group, progesterone group, paclitaxel group, E2（＋）taxol group, and P4（＋）Taxol group was 1.03±0.14,1.60±0.09,1.75±0.16, 1.60±0.10, 1.53±0.06, respectively except estrogen group, significantly higher than that of the control group （ 1.00, P〈0.05）. Relative to the ER （-） in ovarian cancer cells, the Drosha protein expression levels of the control group, estrogen group, progesterone group, paelitaxel group, E2（＋）taxol group, and P4（＋）Taxol group were 0.25±0.05,0.87±0.30,0.85±0.38, 1.30±0.21, 1.75±0.83,1.62±0.82, respectively, with a significant difference between the experimental groups and the

关 键 词：卵巢肿瘤 雌激素 孕激素 紫杉醇 Drosha基因 细胞生长过程 细胞 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]