白细胞介素17A与原发性肝细胞癌上皮间质转化的相关性  

Associations between interleukin-17A expression and epithelial-mesenchymal transition in patients with hepatocellular carcinoma

作　　者：徐军[1] 王健[1] 胡勇[1] 程兰兰[1] 余宏宇[2] 

机构地区：[1]解放军第一○五医院病理科,合肥230031 [2]第二军医大学附属长征医院病理科

出　　处：《中华肿瘤杂志》2015年第8期585-590,共6页Chinese Journal of Oncology

基　　金：南京军区隈学创新课题（14MS036）

摘　　要：目的探讨白细胞介素17A（IL-17A）在原发性肝细胞癌（HCC）组织中的表达及其与上皮间质转化（EMT）和临床病理特征间的联系。方法采用免疫组化和核酸分子原位杂交技术检测HCC组织和癌旁组织中IL-17A蛋白、E-钙黏附蛋白（E—cadherin）、波形蛋白（Vimentin）和SnailmRNA的表达情况。结果HCC组织和癌旁组织中IL-17A阳性率分别为54．1％（40／74）和25．0％（5／20），差异有统计学意义（P〈0．05）。有无门静脉癌栓、有无镜下癌栓、组织学分级、国际抗癌联盟（UICC）分期与IL-17A表达有关（均P〈0．05）。IL-17A阳性HCC患者术后1、2年无瘤生存率分别为27．6％和17．2％，1、2年生存牢分别为69．0％和27．8％；IL-17A阴性HCC患者术后1、2年无瘤生存率分别为79．3％和58．5％，1、2年生存率分别为91．3％和87．0％，差异均有统计学意义（均P〈0．05）。多因素生存分析显示，IL-17A表达水甲和有无镜下癌栓为影响HCC患者无瘤生存时间的独立因素，IL-17A表达水平、有无镜下癌栓和UICC分期为影响HCC患者生存时间的独立因素（均P〈0．05）。Spearman相关分析显示，IL-17A表达与E—cadhefin表达呈负相关（r=-0．404，P〈0．05），与Vimentin和Snail表达呈正相关（r=0．492，P〈0．01；r=0．410，P〈0．05）。结论原发性HCC中IL-17A的表达对EMT有重要影响，可为肝癌的分化、侵袭、转移研究和患者的预后判断提供帮助。Objective To detect the expression of interleukin-17A （IL-17A）in hepatocellular carcinoma （HCCs） tissues, and to analyze its relationship with clinicopathological characteristics and epithelial-mesenchymal transition （EMT）. Methods The expression of IL-17A, E-cadherin, vimentin proteins and Snail mRNA were detected by inmmnohistoehemistry and in situ hybridization histochemistry in the hepatocellular carcinoma （HCC） tissues of 74 patients. Results IL-17A staining was detected in 54.1% （40/74） specimens of human HCCs, but only 25.0% （5/20） in corresponding peritumoral tissues （P〈 0.05）. The positive rate of IL-17A expression in HCC patients with grade Ⅲ＋Ⅳand UICC stage Ⅲ ＋Ⅳ tumors was significantly higher than those with grade Ⅰ ＋ Ⅱ and UICC stageⅠ ＋ Ⅱ tumors. The expression of IL-17A was positively correlated with portal vein tumor thrombus and microvascular invasion（ all P〈0.05 ）. The 1- and 2-year recurrrence-free survival rates were 27.6% and 17.2% in the patients with positive IL-17A expression, but 79.3% and 58.5% in IL-17A-negative HCCs. The 1- and 2-year overall survival rates were 69.0% and 27.8% in the cases with positive IL-17A expression, while 91.3% and 87.0% in IL-17A-negative cases. Patients with IL-17A-pusitive HCCs showed significantly shorter recurrence-free and overall survival compared with the patients with IL-17A-negative HCCs（P〈0.05）. Multivariate analysis indicated that IL- 17A expression was an independent factor for recurrence-free and overall survival of HCCs. IL-17A-positive HCCs were characterized by increased expression of vimentin（r= 0.492, P〈0.01 ） or Snail （r= 0.410, P〈 0.05） and loss of E-cadherin expression（r=-0.404, P〈0.05）. Conclusions Our results suggest that IL- 17A is closely related to epithelial-mcsenchymal transition in hepatocellular carcinoma. IL-17A-positive hepatocellular carcinoma demonstrates more aggressive biological behavior, and IL-17A may serve as apotential prognostic marker fo

关 键 词：肝肿瘤 白细胞介素17A 上皮间质转化 预后 

分 类 号：R735.7[医药卫生—肿瘤]