藜麦皂甙的测定研究  

Determination of Quinoa Saponins

作　　者：任卓伟 倪文杰[2] 刘森[2] 

机构地区：[1]山西卓世鸿基科技有限公司,山西太原030031 [2]山西省农业科学院农产品加工研究所,山西太原030031

出　　处：《山西农业科学》2015年第8期932-935,共4页Journal of Shanxi Agricultural Sciences

摘　　要：用高氯酸比色法对藜麦中皂甙的测定进行了研究,并且测定了水洗后藜麦皂甙的含量变化。结果表明,用香草醛-高氯酸比色法测定藜麦中的皂甙含量是一种方便快捷、结果稳定的方法。测定藜麦皂甙选用齐墩果酸为标准品,波长为560 nm,标准曲线方程为y=0.196 6x-0.013 7,并且R2显示在此范围内有良好的线性关系。提取样品皂甙的条件为:提取液乙醇的体积分数为70%,料液比为1∶30,提取温度为50℃。结果测定的藜麦皂甙含量为10.709 mg/g,水洗之后藜麦中皂甙含量有所减少,清洗的次数越多,藜麦中皂甙的含量越少,清洗3次后藜麦中总皂甙含量由原来的10.709 mg/g降低为6.077 mg/g,减少了43.25%。并且水洗去除藜麦种皮中的皂甙占总去除皂甙的87%。This article with vaniUin-perchloric acid colorimetric method for the determination of saponins in quinoa was studied and measured changes in the content of quinoa saponins after washing. The results showed that using vanillin-perchlorie acid colorimetric method for determination of content of saponins in quinoa stable method was a convenient and quick results. Quinoa saponins determination of oleanolic acid was chosen as the standard, wavelength was 560 nm, the standard curve equation was y = 0.196 6x - 0.013 7, and R2 displayed within this range a good linear relationship. Conditions saponin extract samples were ethanol extract volume fraction of 70%, solid-liquid ratio of 1 ： 30, extraction temperature of 50 ~C. The quinoa saponins determination was 10.709 mg/g, after washing quinoa saponin content decreased, the more cleaning the number, the less saponin content of quinoa, and washed three times in quinoa saponins original content the 10.709 mg/g reduced to 6.077 mg/g, reducing 4.632 mg/g, accounting for 43.25% of the original content. Water removed the saponins in the seed coat 87% of the total saponin saponins.

关 键 词：藜麦 皂甙 含量测定 比色法 齐墩果酸 

分 类 号：S512.9[农业科学—作物学]