p21-活化激酶1靶向siRNA协同5-氟尿嘧啶对大肠癌细胞化疗增敏作用  

Effect of chemotherapy sensitivity enhancement by RNA interference targeting p21-activated kinase 1 with 5-fluorouracil on human colon cancer cells

作　　者：青海涛[1] 龚伟[1] 刘思德[1] 

机构地区：[1]南方医科大学南方医院消化内科,广州市510515

出　　处：《实用医学杂志》2015年第16期2598-2601,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助(编号:81201662)

摘　　要：目的:探讨大肠癌细胞中p21-活化激酶1(PAK1)对5-氟尿嘧啶(5-FU)化疗敏感性的影响。方法:CCK8法测定增殖;流式细胞仪测定凋亡率;Hoechst染色法检测凋亡;Western Blot检测Bcl-xl、Bcl-2、XIAP表达。结果 :敲除PAK1可抑制Lo Vo细胞的生长,而5-FU联合sh RNA-PAK1组(联合组)细胞增殖受到最显著抑制(P<0.05)。各组凋亡率:联合组(53.36±4.03)%、sh RNA-PAK1转染组(26.48±2.78)%、5-FU组(15.52±1.30)%,联合组凋亡率显著增高(P<0.05)。联合组凋亡核所占比率即凋亡率最高(P<0.01)。联合组Bcl-xl、Bcl-2、XIAP的表达较单独5-FU组及sh RNA-PAK1组抑制更加明显。结论 :RNA干扰抑制PAK1表达后Lo Vo细胞对5-FU的敏感性增加,更显著抑制增殖、诱导凋亡。Objective To investigate the effect of p21-activated kinase 1 on chemotherapy sensitivity of 5-fluorouraeil. Methods Cell proliferation was measured by CCK8 and apoptosis rate by flow eytometry or Hoechst staining; the expression of Bcl-xl, Bcl-2, XIAP were determined by Western Blot. Results 5-FU combined shRNA-Pak1 group （combination group） could be significantly inhibited in terms of proliferation （P 〈 0.05）. The percentage of apoptosis rate in combined group was the highest and the difference among groups indicated statistical significance （P 〈 0.05）. The expression of Bcl-xl, Bcl-2, XIAP in combination group was significantly inhibited compared with 5-FU group or shRNA-Pak1 group. Conclusion PAK1 inhibited by RNA interference can enhance chemotherapy sensitivity of 5-Fu on growth inhibition and apoptosis induction in colon cancer significantly.

关 键 词：大肠肿瘤 p21-activatedkinase1 5-氟尿嘧啶 化疗增敏 

分 类 号：R735.34[医药卫生—肿瘤]