DcR3 RNA干扰对卵巢癌细胞株CAOV3增殖能力的影响  

Effect of RNA Dc R3 interference on the proliferation of ovarian cancer cell CAOV3

作　　者：林静娴[1] 彭勇[1] 遇桂芳[1] 曾琼[1] 钟婷[1] 

机构地区：[1]暨南大学附属珠海市人民医院妇科,广东省珠海市519000

出　　处：《实用医学杂志》2015年第16期2601-2604,共4页The Journal of Practical Medicine

基　　金：广东省医学科研基金(编号:B2013364)

摘　　要：目的:利用RNA干扰(RNAi)技术沉默卵巢癌细胞系CAOV3细胞中诱骗受体3(Dc R3)基因的表达,并探讨其对卵巢上皮性腺癌(卵巢癌)细胞增殖能力的影响。方法:构建Dc R3 si RNA,并转染至卵巢癌CAOV3细胞中,在细胞内被识别并降解CAOV3细胞中的Dc R3 m RNA。实验分为3组:Dc R3 si RNA组、空白对照组和非特异性si RNA组(阴性对照组)。通过实时荧光定量(Real-time PCR,RT-PCR)检测Dc R3m RNA表达水平的变化。利用MTT实验观察Dc R3 si RNA对卵巢癌细胞株CAOV3增殖能力的影响。结果 :Dc R3 si RNA组细胞中Dc R3 m RNA的表达水平明显降低,与非特异性si RNA组及空白对照组相比,差异有显著性(P<0.05)。转染Dc R3 si RNA的细胞增殖能力明显下降,与非特异性si RNA组及空白对照组相比,差异有统计学意义(P<0.01)。结论:Dc R3 si RNA能在细胞内被识别并降解CAOV3细胞中的Dc R3 m RNA,从而发挥抑制卵巢癌细胞增殖的能力。Objective To investigate the effects of siRNA targeting decoy receptor 3 on the cell proliferation of ovarian carcinoma cell CAOV3. Methods We constructed siRNA targeting decoy receptor 3,which was transfected into ovarian carcinoma cells CAOV3, and observed the effects of DcR3 siRNA on the cell proliferation of CAOV3 cell by MTT experiment. The experiment contained 3 groups, including the normal control group （CAOV3 cell was not transfected）, the negative control group （CAOV3 cell was transfected with blank vector） and the experimental group （CAOV3 cell was transfected with DcR3 siRNA）. The expression levels of DcR3 mRNA were detected by Real-time PCR. Results DcR3 siRNA recognized and degraded DcR3 mRNA in CAOV3 cells of the experimental group. DcR3 mRNA of the experimental group was significantly decreased. The proliferation of CAOV3 cell was significantly decreased by DcR3 siRNA comparing with the normal control group and negative control group （P 〈 0.01）. Conclusion DcR3 siRNA can inhibit the proliferation of ovarian cancer cell line CAOV3 by recognized and degraded DcR3 mRNA.

关 键 词：诱骗受体3 RNA干扰 卵巢癌细胞 增殖能力 

分 类 号：R737.31[医药卫生—肿瘤]