胡柚小青果的黄酮类化合物及抗氧化活性研究  

Flavonoids contents and antioxidant activities in Citrus paradisi cv. Changshan Huyou during development

作　　者：郑雪良 刘春荣 王登亮 毕旭灿 杨波 孙崇德[3] 

机构地区：[1]浙江省衢州市农业科学研究院,浙江衢州324000 [2]浙江省常山县农业局,浙江常山324200 [3]浙江大学果实品质生物学实验室,浙江杭州310058

出　　处：《浙江农业学报》2015年第7期1185-1191,共7页Acta Agriculturae Zhejiangensis

基　　金：衢州市科技计划项目(2011Z001);浙江省重大科技专项子专题(2012C12904-1)

摘　　要：以中药枳壳为对照,以不同发育阶段胡柚小青果为试材,采用HPLC技术检测黄酮类化合物含量的变化,以DPPH和FRAP两种抗氧化方法检测抗氧化活性。结果表明,胡柚小青果的总黄酮含量高,5个时期的含量分别为173.86,150.06,134.43,65.33,76.60 mg·g-1,枳壳中含量为85.65 mg·g-1。胡柚小青果和枳壳的黄酮类化合物绝大部分以黄烷酮糖苷形式存在,主要为柚皮苷,其次为新橙皮苷,还含有柚皮芸香苷、圣草次苷、新圣草次苷、六甲氧基黄酮、七甲氧基黄酮和橘皮素等。胡柚小青果的抗氧化活性显著高于枳壳,其中以6月11日的胡柚小青果抗氧化活性最高。Citrus paradisi cv. Changshan Huyou and Fructus aurantii were used as materials, flavonoids contents were determineds by HPLC and antioxidant activitie were evaluated by methods of FRAP and DPPH. Huyou fruits has a higher content of total flavonoids, the contents in five periods of Huyou fruits were 173.86, 150. 06, 134. 43, 65.33 and 76. 60 mg＇g-~ ; meanwhile, the content of total flavonoids in Fructus aurantii was 85.65 mg.g-~. Flava- none glycosides are the main fiavonoids in fruits of Fructus aurantii and Huyou, the main flavonoids were naringin and hesperidin, followed by neohesperidin, narirutin, neoericitrin, ericitrin, hexaMF, heptaMF and tangeretin ect. The antioxidant activities of Huyou was significantly higher than that of Fructus aurantii, and the fruits of Huyou on June 11 th had the highest antioxidant activities.

关 键 词：胡柚小青果 枳壳 黄酮类化合物 抗氧化活性 

分 类 号：S666.9[农业科学—果树学]