喜树碱对番茄灰霉病菌的毒理效应  

Toxicological effects of camptothecin to Botrytis cinerea

作　　者：毛胜凤[1,2] 周湘[2] 范闻春 张立钦[2] 叶建仁[1] 

机构地区：[1]南京林业大学林学院南方现代林业协同创新中心,江苏南京210037 [2]浙江农林大学林业与生物技术学院,浙江临安311300

出　　处：《浙江农林大学学报》2015年第4期585-589,共5页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金资助项目(LY14C140005);浙江农林大学大学生创新训练项目(201302021)

摘　　要：为探明喜树碱对番茄灰霉病菌Botrytis cinerea的毒理效应,应用生长速率法、电子显微镜观察研究不同质量浓度喜树碱对番茄灰霉病菌菌丝生长、细胞壁结构的影响;番茄Lycopersicon esculentum叶片经喜树碱处理后接种番茄灰霉病菌,观察药剂对病菌侵染活性的影响。结果表明:喜树碱为3.13 mg·L-1时就能强烈抑制番茄灰霉病菌菌丝生长,抑菌率达45.49%,差异显著(P<0.05);可显著影响番茄灰霉病菌对番茄活体叶片的侵染活性;喜树碱处理可导致菌体细胞壁表面出现破损、穿孔,细胞内含物外渗等现象。喜树碱能破坏菌丝体细胞结构,从而抑制番茄灰霉病菌的生长,而在活体叶片可显著影响病菌的侵染活性。In order to ascertain the toxicology effects of camptothecin to Botrytis cinerea, growth rates were de- termined by the application of different concentrations of camptothecin inhibition of mycelial growth and cell well structure of B. cinerea. The results showed that camptothecin concentration 3.13 mg.L-1 can strongly in- hibit the mycelial growth, inhibition rate 45.49% antibacterial rate of significant differences. In the scanning electron microscope, camptothecin treated by B. cinerea, mycelial cells rupture, perforation, shriveled, growing point of collapse, cytoplasmic extravasation. In vivo effects of camptothecin on B. Cinerea showed that camp- tothecin against B. cinerea has a significant protective effect.

关 键 词：植物保护学 喜树碱 番茄灰霉病菌 毒理效应 

分 类 号：S482.2[农业科学—农药学]