混合胶束毛细管电动色谱法测定片仔癀中有效成分  

Determination of Active Components in Pien Tze Huang by Mixed Micellar Electrokinetic Chromatography

作　　者：余丽双[1] 丛日琳 林诗瑶 孙照霞[1] 李煌[1] 林埔[1] 褚克丹[1] 徐伟[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]荣成市中医院,山东威海264300

出　　处：《分析科学学报》2015年第4期505-508,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.81202913);福建省科技厅社会发展重点项目(No.2013Y0057);福建省卫生系统中青年骨干人才培养项目(No.2014-ZQN-JC-27);福建省教育厅资助项目(No.JK2012023)

摘　　要：采用混合胶束毛细管电动色谱法(mixMs-MEKC)同时分离检测片仔癀中的三七皂苷R1、人参皂苷Rg1、麝香草酚、人参皂苷Rb1及牛磺胆酸钠5种有效成分。实验发现,采用单一胶束5种目标化合物不能完全分离,当采用胆酸钠-十二烷基硫酸钠(SC-SDS)二元混合胶束时5种化合物达到完全分离。实验优化了混合表面活性剂的浓度、缓冲溶液pH值、分离电压和进样时间等条件对分离效果的影响。在最优化的电泳条件下,5种化合物在16min内得到基线分离,且线性关系良好,检测限(S/N=3)分别为0.2μg/mL、0.08μg/mL、0.01μg/mL、0.2μg/mL、0.2μg/mL。将该方法应用于片仔癀中上述化合物含量的测定。An efficient method of mixed micellar electrokinetic chromatography（MEKC）was established to determine notoginsenoside R1,gensenoside Rg1,thymol,ginsenoside Rb1 and sodium taurocholate in Pien Tze Huang.The results indicated that the five analytes could not be separated in single micelle,but could be completely separated in mixed micelles.The optimal separation conditions were obtained by discussing the pH of buffer,concentration of mixed micellar,separation voltage and injection time.Under the optimal conditions,the five analytes could be completely separated and detected within 16 min with good linearity,and the detection limits were 0.2,0.08,0.01,0.2and 0.2μg/mL,respectively.The proposed method was applied for the determination of the active components in Pien Tze Huang with satisfactory results.

关 键 词：混合胶束 毛细管电动色谱法 三七皂苷 人参皂苷 片仔癀 

分 类 号：O657.8[理学—分析化学]