黄奇楠生物活性和化学成分的研究  

Bioactivities and chemical constituents of Huangqi'nan

作　　者：杨锦玲[1,2] 梅文莉[1] 蔡彩虹[1] 董文化[1] 陈亮亮[1] 戴好富[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南省沉香工程技术研究中心,海南海口571101 [2]海南大学园艺园林学院,海南海口570228

出　　处：《中成药》2015年第8期1740-1747,共8页Chinese Traditional Patent Medicine

基　　金：公益性行业(农业)科研专项(201303117);国家科技支撑计划课题项目(2013BAI11B04);海南省重大科技项目(ZDZX2013013)

摘　　要：目的研究黄奇楠的生物活性,并分析其化学成分。方法对黄奇楠乙醚提取物进行抗菌、抗肿瘤、乙酰胆碱酯酶抑制和降血糖活性测试,然后应用HP6890/5975C GC/MS联用仪分析其化学成分。结果乙醚提取物对金黄色葡萄球菌、慢性髓原白血病细胞K562、人胃癌细胞SGC-7901、乙酰胆碱酯酶和α-葡萄糖苷酶均表现出较好的抑制活性。同时,GC-MS检测到30个成分(相对含有量为91.46%),并鉴定了其中26个化合物,包括16个倍半萜类成分(相对含有量为52.94%)和3个2-(2-苯乙基)色酮类成分(相对含有量为16.09%)。其中,有7个倍半萜具有香味(相对含有量为36.29%)。结论黄奇楠乙醚提取物具有抗菌、抗肿瘤、乙酰胆碱酯酶抑制和降血糖活性,并含有多种化学成分,主要为倍半萜类型。AIM To study the bioactivities and chemical constituents of Huangqi ＇nan ethereal extract.METHODS The antibacterial,antitumor,acetylcholinesterase（ ACh E） inhibitory and hypoglycemic activities of Huangqi＇nan ethereal extract were tested. Then the chemical constituents of this plant were analyzed by HP6890 /5975 C GC-MS. RESULTS The Huangqi＇nan ethereal extract showed antibacterial activity toward Staphylococcus aureus,antitumor activity toward chronic myelogenous leukemia cells K562 and human gastric carcinoma cells SGC-7901,ACh E inhibitory activity and α-glucosidase inhibitory activity. Meanwhile,thirty compounds（ 91. 46%）,including 16 sesquiterpenes（ 52. 94%） and three 2-（ 2-phenylethyl） chromone derivatives（ 16. 09%） were identified. Among them,seven sesquiterpenes were reported to be fragrant（ 36. 29%）. CONCLUSION The Huangqi＇nan ethereal extract has antibacterial,antitumor,ACh E inhibitory and hypoglycemic activities,and contains various chemical constituents,mainly of which are sesquiterpenes.

关 键 词：黄奇楠 乙醚提取物 抗菌 抗肿瘤 乙酰胆碱脂酶抑制 降血糖 GC-MS 化学成分 

分 类 号：R284.1[医药卫生—中药学]