裙带菜孢子叶多糖的超声辅助提取工艺优化及其抗氧化活性研究  

Study on polysaccharide ultrasonic-assisted extraction process and antioxidant activity in Sporophyll of Undaria pinnatifida

作　　者：董秀芳[1,2] 李楠[1,2] 韩冬[1] 冯丁丁[1,2] 傅卉[1,2] 焦阳[1] 熊欣[1] 启航[1,2] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]国家海洋食品工程技术研究中心,辽宁大连116034

出　　处：《食品与机械》2015年第4期162-166,177,共6页Food and Machinery

基　　金：国家高技术研究发展计划(编号:2014AA093602);辽宁省农业领域青年科技创新人才培养计划(编号:2014001);国家海洋食品工程技术研究中心立项建设项目(编号:2012FU125X03)

摘　　要：为优化超声波辅助提取裙带菜孢子叶多糖工艺,探究其抗氧化活性。通过单因素试验考察各因素对多糖得率的影响,采用Box-Behnken设计方法建立多糖提取的教学模型;同时利用电子自旋共振技术探究裙带菜孢子叶多糖的抗氧化活性。结果表明:超声波辅助提取裙带菜孢子叶多糖的最优条件为液料比90∶1(V∶m),超声时间6.5min,超声功率440 W,提取时间2h,该条件下测得多糖得率为7.71%。孢子叶粗多糖对DPPH·和·OH具有较好的清除能力,其IC50分别为0.24,0.28mg/mL。Polysaccharide yield was as index, and optimal ultrasonic assisted extraction process of polysaccharides from sporophyll of Un- daria pinnatifida was investigated by Box-Behnken design based on single factor tests. Meanwhile, the antioxidant activity of the poly saccharide was also studied by electron spin resonance （ESR） tech- nology. It turned out that the optimal extraction conditions were as follow： ratio of water to material was 90 ： 1 （V ： m）, ultrasonic time was 6.5 min and ultrasonic power was 440 W, extraction time was 2 h, respectively. Polysaccharide yield achieved 7. 71% underthat condition. And the polysaccharide from sporophyll of Undaria pinnatifida gave potent scavenging activity against DPPH · and · OH, the IC50 were 0.24 mg/mL and 0.28 mg/mL, respectively.

关 键 词：裙带菜 孢子叶 多糖 超声波 抗氧化活性 电子自旋共振技术 

分 类 号：TQ281[化学工程—有机化工]