肝硬化患者HBV血清标志物模式与HBV-DNA定量检测结果分析  

Analysis the mode of HBVM and the detection of HBV-DNA in patients with liver cirrhosis

作　　者：肖春梅 贺岩 何瑞芬 张俊华 朴文花 

机构地区：[1]宁夏人民医院临床医学检验诊断中心,宁夏银川750002

出　　处：《宁夏医学杂志》2015年第8期699-700,共2页Ningxia Medical Journal

基　　金：宁夏卫生厅科研计划资助项目(2012104)

摘　　要：目的探讨肝硬化患者血清标志物模式（HBV-M）与病毒复制指标HBV-DNA相关性及意义,为临床治疗肝硬化提供依据。方法分别利用化学发光法和荧光定量PCR法对138例肝硬化患者血清进行HBVM和HBV-DNA定量测定。结果 138例肝硬化患者中HBV-M感染模式有9种,HBe Ag阴性者检出率为69.5%,高于HBe Ag阳性者;HBe Ag阴性组和HBe Ag阳性组HBV-DNA阳性率分别为62.5%、71.5%,HBe Ag阴性组HBV-DNA含量103~104IU·m L-1构成比为43.8%（42/96）,105~107IU·m L-1构成比为18.7%（18/96）;HBe Ag阳性组HBV-DNA含量103~104IU·m L-1构成比为21.4%（9/42）,105~107IU·m L-1构成比为50.1%（21/42）。结论乙型肝炎肝硬化患者以HBe Ag阴性及HBV-DNA阳性居多,临床应高度重视,早期应进行积极有效的HBV抗病毒治疗。Objective To explore the relationship between hepatitis B virus markers （HBVM） and HBV - DNA quantification in order to provide evidence for clinical therapy of liver cirrhosis. Methods HBVM for138 samples obtained from routine work in clini- cal laboratory were investigated by time - resolution immunofluorescence. HBV - DNA quantity of those samples were detected with the real time fluorescent quantitative PCR instrument. Results 138 samples tested were divided into 11 models. The infection patterns con- taining negative HBeAg were the most,which accounted for 69.5%. The positive rates of HBV - DNA in negative and positive HBeAg patients were 62.5 % and 71.5 %, respectively. In samples with different levels of HBV - DNA, constituent ratios in serum had significant difference （P 〈 0.05 ）. The percentage of the positive cases （ HBV - DNA = 103 ~ 104 copies ~ mL-l ） was 43.8 % （42/96） in HBeAg negative group and 21.4% （9/42） in HBeAg positive group. However,the percentage of the positive cases （ HBV - DNA = 105 ~ l0T copies ~ mL-1 ） was 18.7% （18/96） in HBeAg negative group and 50.1% （21/42） in HBeAg positive group. Conclusion The liver cirrhosis is closely related to HBV infection in which negative HBeAg was predominant. Reflection of HBV - DNA in liver cirrhosis pa- tient blood can reflect the degree of HBV duplication and activeness in those patients,which have an important value on clinical judge- ment of liver cirrhosis degree and guiding anticirrhosis.

关 键 词：肝硬化 乙型肝炎病毒 血清标志物 乙肝病毒核酸 

分 类 号：R575.2[医药卫生—消化系统]