高分辨率熔解曲线分析法检测结直肠癌组织K-ras基因突变及其临床意义  

High-resolution melting analysis for detection of K-ras mutation in colorectal cancer and its clinical significance

作　　者：王倩[1] 顾页[1] 林洁[1] 

机构地区：[1]南方医科大学基础医学院病理学系,广州医学硕士研究生510515

出　　处：《医学研究生学报》2015年第8期840-842,共3页Journal of Medical Postgraduates

基　　金：广州市科技计划项目(2012J2200044)

摘　　要：目的 K-ras基因在结直肠癌的发生、发展以及需要靶向药物治疗的药物选择方面具有重大临床意义。文中旨在探讨高分辨率熔解曲线分析法检测结直肠癌组织K-ras基因突变情况,并分析K-ras基因突变与临床病理参数的关系。方法收集179例结直肠癌患者的手术标本,检测结直肠癌组织中K-ras基因第12、13位密码子的突变情况,并分析K-ras基因突变与临床病理参数的关系。结果 179例结直肠癌组织标本中,K-ras基因突变率为43.02%（77/179）;在≥60岁组结直肠癌患者中K-ras基因突变率明显高于〈60岁组（55.17%vs 33.70%,P〈0.05）。多因素Logistic回归分析结果显示,年龄对K-ras基因突变有显著影响（OR=1.506,95%CI：1.028~2.011）。结论结直肠癌患者K-ras基因的突变与年龄相关,与性别、分化程度、病变部位及Dukes分期无关。Objective The K-ras gene plays a major role in the development, progression, and drug selection for the target treatment of colorectal cancer. The aim of this study was to evaluate the feasibility of detecting K-ras mutation in coloreetal cancer by high-resolution melting （HRM） analysis and to investigate the relationship between K-ras mutation and the clinicopathological parame- ters of colorectal cancer. Methods We collected the tissue samples of colorectal cancer from 179 patients, detected the mutations in codons 12 and 13 of the K-ras gene, and analyzed the relationship between K-ras mutation and the clinicopathological parameters of the patients. Results In the 179 cases of colorectal cancer, K-ras mutation was found in 77 （43.02%） , significantly higher in those aged t〉60 years than in those aged 〈 60 years （55.17% vs 33.70%, P 〈 0.05 ）. Multivariate logistic regression analysis showed a significant influence of age on K-ras mutation （OR=1.506, 95%CI： 1.028 -2.011, P〈0.05）. Conclusion HRM analysis is a rapid, sensitive, and inexpensive diagnostic tool for the detection of K-ras mutation, and K-ras mutation is associated with the age of colorectal cancer patients.

关 键 词：高分辨率熔解曲线分析法 结直肠癌 K-RAS基因 突变 

分 类 号：R656.9[医药卫生—外科学]