IGF-Ⅱ促进人肝癌细胞MHCC97-H生长增殖作用的研究  

The promotion of IGF-Ⅱ on growth and proliferation in MHCC97-H cells

作　　者：姬媛媛[1] 王志东[2] 杨正安[2] 王宝太[2] 史敏[2] 徐慧荔 赵敏侠 

机构地区：[1]西安交通大学第二附属医院科研中心实验室,陕西西安710004 [2]西安交通大学第二附属医院干四病区,陕西西安710004

出　　处：《现代肿瘤医学》2015年第17期2399-2403,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81201925);西安交通大学第二附属医院人才培养专项科研基金资助项目[编号:RC(GG)201404]

摘　　要：目的:探讨IGF-Ⅱ在人肝癌细胞MHCC97-H生长增殖中的作用。方法:培养人肝癌细胞株MHCC97-H,IGF-Ⅱ(25,50,100ng/ml)作用于不同时间点(24、48、72h),观察各组细胞形态变化,噻唑兰(MTT)法分析细胞生长情况,Brdu法检测细胞增殖潜能,平板克隆实验观察并计算细胞克隆形成率,细胞免疫荧光及Western blot法观察细胞增殖核抗原(PCNA)表达,进一步分析细胞增殖情况。结果:IGF-Ⅱ可使MHCC97-H细胞形态发生一定改变;IGF-Ⅱ时间依赖性地增加MHCC9 7-H细胞存活率,但与对照组比较无显著差异(P>0.05);IGF-Ⅱ可促进MHCC97-H细胞增殖,但与对照组比较无显著差异(P>0.05);IGF-Ⅱ(50ng/ml)对MHCC97-H细胞的克隆形成有一定的促进作用,但与对照组比较无显著差异(P>0.05);与对照组相比,IGF-Ⅱ(50ng/ml)组,MHCC97-H细胞中PCNA荧光表达和蛋白表达显著升高(P<0.05)。结论:IGF-Ⅱ有诱导人肝癌细胞MHCC97-H生长增殖的趋势,亦可促进PCNA表达上调。Objective：To investigate the effect of IGF -II on growth and proliferation in MHCC97 -H cells. Methods：Morphology of cells was observed under microscope. Relative viability（ MTT-assay）of MHCC97-H cells was determined after application of IGF-II（25,50,100 ng/ml）at different time points（24,48,and 72h）. Relative proliferation（BrdU-assay）of MHCC97-H cells was measured 48h after application of IGF-II. The clone formation efficiency of cells was determined by plate clone formation experiment. Expression of characteristic markers of prolifer-ation（ PCNA ）was identified with immunofluorescence. Furthermore,protein expression of PCNA was detected by Western blot. Results：MHCC97 -H cells induced by IGF -II showed a morphological alteration. MTT,BrdU and clone-formation assay suggested that growth and proliferation of MHCC97-H cells were increased by IGF-II,but no significant difference was found between IGF-II groups compared with control group（P〉0. 05）. Moreover,com-pared with the control,immunofluorescent expression of PCNA was evidently enhanced by IGF-II（P〈0. 01）. And protein expression of PCNA was significantly increased by IGF-II（P〈0. 05）. Conclusion：IGF-II might induce growth and proliferation and up-regulate PCNA expression trend in MHCC97-H cells.

关 键 词：生长 增殖 MHCC97-H细胞 

分 类 号：R730[医药卫生—肿瘤] R735.7[医药卫生—临床医学]