鼻咽癌组织中磷酸果糖激酶1蛋白表达及其酶活性检测  

Expression of phosphofructokinase 1 protein and it′s enzyme activity on nasopharyngeal carcinoma

作　　者：李烁[1] 洪海裕[2] 杜政德[1] 刘飞[1] 杨琼[1] 高春生[1] 

机构地区：[1]广东医学院附属深圳南山医院耳鼻咽喉科,深圳518052 [2]中山大学附属第五医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第16期1455-1457,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：深圳市南山区科技局基金(No:2012031)

摘　　要：目的:研究糖酵解途径关键酶磷酸果糖激酶1(PFK1)的蛋白及其酶活性在鼻咽癌活检组织中的表达情况,并探讨其意义。方法:采取病例-对照设计的方法 ,实验组为41份鼻咽癌患者的鼻咽癌活检组织标本,对照组为20份鼻咽慢性炎性患者的鼻咽部活检组织标本,通过Western blot检测PFK1蛋白的表达;酶学分析的方法检测PFK1酶活性。结果:实验组的PFK1蛋白表达及其酶活性水平均明显高于对照组(P<0.01);在实验组中,伴有淋巴结转移的鼻咽癌活检标本中的PFK1蛋白及其酶活性表达水平均明显高于无淋巴结转移的鼻咽癌活检标本(P<0.01)。结论:PFK1与鼻咽癌的发生、发展密切相关,可能作为鼻咽癌的肿瘤标志物之一。Objective：The aim of this study is to investigate the expression of phosphofructokinase 1 and it＇s enzyme activity in nasopharyngeal carcinoma biopsy samples. Method：Sixty-one biopsy samples were detected, in- cluding 41 tissues from patients with nasopharyngeal carcinoma as experimental group and 20 tissues from patients with chronic nasopharyngitis as control group. Phosphofructokinase 1 protein was detected by Western blot and it＇s enzyme activity was detected. Resuit：It was observed that the expression levels of phosphofruetokinase 1 pro- tein and itls enzyme activities in the experimental group were higher than that in the control group （P〈0. 01）. In the experimental group, the expression levels of phosphofructokinase 1 protein and it＇s enzyme activities in patients with lymph node metastasis were higher than that in patients without lymph node metastasis（P〈0.01）. Conclusion：Phosphofructokinase 1 may be a marker in occurrence and metastasis of nasopharyngeal carcinoma.

关 键 词：磷酸果糖激酶1 鼻咽肿瘤 糖酵解 淋巴结 

分 类 号：R739.63[医药卫生—肿瘤]