白色念珠菌感染对口腔白斑中RIP2和Caspase-12表达的影响  

Effects of Candidal albicans infection on expression of RIP2 and Caspase-12 in oral leukoplakia

作　　者：蒋文晖[1,2] 王文梅[1,2] 王翔[1,2] 钱雅洁[1,2] 高雅凡 段宁[1,2] 蒋红柳[1,2] 白杨[1,2] 

机构地区：[1]南京大学医学院附属口腔医院 [2]南京市口腔医院,江苏南京210008

出　　处：《临床口腔医学杂志》2015年第8期469-472,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金项目(81070839);江苏省临床医学科技专项(BL2014018);南京市科技计划项目(201402033)

摘　　要：目的:研究非白色念珠菌口腔白斑和白色念珠菌口腔白斑组织中RIP2、Caspase-12的表达,探讨白色念珠菌感染对口腔白斑组织中RIP2和Caspase-12表达的影响。方法:收集40例口腔白斑组织,通过HE染色、真菌银染法和免疫组化法检测白色念珠菌抗原确定是否伴有白色念珠菌感染,采用免疫组化法检测非白色念珠菌白斑和白色念珠菌白斑组织中RIP2和Caspase-12的表达,图像分析测定平均光密度值,通过统计分析评价表达水平的差异。结果:白色念珠菌白斑组中RIP2和Caspase-12的表达水平较非白色念珠菌白斑组明显升高,差异均具有统计学意义(P<0.05)。结论:白色念珠菌感染可能增强口腔白斑组织中RIP2和Caspase-12的表达。Objective：The objective of the study was to investigate the differential expression of RIP2 and Caspase-12 in oral leukoplakia with or without Candida albicans infection. Method：Hematoxylin-eosin staining,silver staining and immunohistochemical staining were adopted to determine Candida albicans infection in 40 different cases of oral leukoplaki-a. Immunohistochemistry was used to investigate the expression of RIP2 or Caspase-12 in oral leukoplakia with or without Candida albicans infection. Then Image J software was employed to analyze the main optical density of immunohistochemical images,which represented the levels of RIP2 or Caspase-12. Result：The levels of RIP2 and Caspase-12 expressed in can-dida albicans leukoplakia were both higher than those in leukoplakia without candida albicans infection（P〈0.05）. Conclu-sion：Candida albicans infection may induce the expression of RIP2 and Caspase-12 in oral leukoplakia.

关 键 词：白色念珠菌 口腔白斑 受体相互作用蛋白2 CASPASE-12 

分 类 号：R781.5[医药卫生—口腔医学]